Role of phosphatidylinositol 3-kinase (PI3K) and Akt1 kinase in porcine reproductive and respiratory syndrome virus (PRRSV) replication

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• 作者：Xiuqing Wang (1)
  Hanmo Zhang (1)
  Alex M. Abel (1)
  Alan J. Young (2)
  Liji Xie (3)
  Zhixun Xie (3)
  
• 关键词：Porcine reproductive and respiratory syndrome virus (PRRSV) ; Phosphatidylinositol ; 3 ; kinase (PI3K) ; Akt1 ; Viral gene transcription ; Viral protein synthesis
• 刊名：Archives of Virology
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：159
• 期：8
• 页码：2091-2096
• 全文大小：370 KB
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• 作者单位：Xiuqing Wang (1)
  Hanmo Zhang (1)
  Alex M. Abel (1)
  Alan J. Young (2)
  Liji Xie (3)
  Zhixun Xie (3)
  
  1. Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA
  2. Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, 57007, USA
  3. Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, Guangxi, 530001, People鈥檚 Republic of China
  
• ISSN：1432-8798

文摘

We have previously reported that inhibition of phosphatidylinositol 3-kinase (PI3K) reduces porcine reproductive and respiratory syndrome (PRRSV) replication. Here, we further investigate the mechanism by which PI3K inhibition affects virus replication and the role of Akt1 kinase in virus replication. We found that PI3K inhibition reduced viral gene transcription by approximately 1.5-fold. Accordingly, viral protein synthesis was significantly reduced by PI3K inhibition. Interestingly, cells overexpressing the dominant negative mutant Akt1 exhibited a significant reduction in viral gene transcription compared to cells overexpressing the constitutively active Akt1. Viral protein synthesis was also enhanced in cells overexpressing the constitutively active Akt1. Overall, our data show that both PI3K and Akt1 play a role in viral gene expression, leading to an increase in virus replication.