Olfactory Ensheathing Cell-Conditioned Medium Protects Astrocytes Exposed to Hydrogen Peroxide Stress

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• 作者：Liu Jinbo (1)
  Liu Zhiyuan (1)
  Zhang Zhijian (2)
  Ding WenGe (1)
  
• 关键词：Olfactory ensheathing cell ; Astrocytes ; H2O2 ; Apoptosis
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2013
• 出版时间：July 2013
• 年：2013
• 卷：33
• 期：5
• 页码：699-705
• 全文大小：542KB
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• 作者单位：Liu Jinbo (1)
  Liu Zhiyuan (1)
  Zhang Zhijian (2)
  Ding WenGe (1)
  
  1. Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou, 213003, Jiangsu, China
  2. School of Medicine, Jiangsu University, Zhenjiang, 212013, China
  
• ISSN：1573-6830

文摘

The purpose of this study was to observe the effects of olfactory ensheathing cell conditioned medium (OECCM) on damaged astrocytes after exposure to H2O2 in vitro. OECCM was used to treat astrocytes after injury, which was induced by exposure to 500?μmol/L H2O2 for 20?min. The cell morphology was then observed under a light microscope, cell viability assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell ultrastructure observed with transmission electron microscopy (TEM), and apoptosis assessed by Annexin V staining followed by cytometry and Western blot. H2O2 induced severe damage to astrocytes as evidenced by decreased cell number, pathological changes in cell morphology, and significantly elevated cell apoptosis. Cells incubated with OECCM displayed significantly improved cell viability and decreased cell apoptotic rate. Under TEM, H2O2-treated cells showed partially broken plasma membranes, swollen rough endoplasmic reticula, visible vacuoles, and swollen or deformed mitochondria with ruptured cristae. Incubation with OECCM significantly ameliorated these pathological changes in astrocytes. These results suggest that OECCM may protect astrocytes from oxidative damage by promoting cell survival while reducing apoptosis of the damaged cells.