Rho-Associated Kinase Inhibitors Promote Microglial Uptake Via the ERK Signaling Pathway

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• 作者：Peicai Fu ; Ronghua Tang ; Zhiyuan Yu ; Caihong Li ; Xue Chen…
• 关键词：Microglia ; Rho ; associated kinase ; Uptake activity ; Extracellular ; signal ; regulated kinase
• 刊名：Neuroscience Bulletin
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：32
• 期：1
• 页码：83-91
• 全文大小：1,464 KB
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• 作者单位：Peicai Fu (1)
  Ronghua Tang (1)
  Zhiyuan Yu (1) (2)
  Caihong Li (1)
  Xue Chen (1)
  Minjie Xie (1) (2)
  Wei Wang (1) (2)
  Xiang Luo (1)
  
  1. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China
  2. Key Laboratory of Neurological Diseases (HUST), Ministry of Education of China, Wuhan, 430030, China
  
• 刊物主题：Neurosciences; Human Physiology; Anesthesiology; Anatomy; Neurology; Pain Medicine;
• 出版者：Springer Berlin Heidelberg
• ISSN：1995-8218

文摘

Microglia are immunocompetent cells in the central nervous system that take up tissue debris and pathogens. Rho-associated kinase (ROCK) has been identified as an important regulator of uptake, proliferation, secretion, and differentiation in a number of cell types. Although ROCK plays critical roles in the microglial secretion of inflammatory factors, migration, and morphology, its effects on microglial uptake activity have not been well characterized. In the present study, we found that treatment of BV2 microglia and primary microglia with the ROCK inhibitors Y27632 and fasudil increased uptake activity and was associated with morphological changes. Furthermore, western blots showed that this increase in uptake activity was mediated through the extracellular-signal-regulated kinase (ERK) signaling cascade, indicating the importance of ROCK in regulating microglial uptake activity. Keywords Microglia Rho-associated kinase Uptake activity Extracellular-signal-regulated kinase