Fullerene (C60) nanoparticles exert photocytotoxicity through modulation of reactive oxygen species and p38 mitogen-activated protein kinase activation in the MCF-7 cancer cell line

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• 作者：Zhi Li (1)
  Fei-long Zhang (1)
  Zhiyuan Wang (1)
  Li-li Pan (1)
  Ying-ying Shen (1)
  Zhen-zhong Zhang (1)
  
• 关键词：Fullerene (C60) ; Photocytotoxicity ; Cell cycle ; p38MAPK ; ROS ; Health and safety effects
• 刊名：Journal of Nanoparticle Research
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：15
• 期：12
• 全文大小：
• 作者单位：Zhi Li (1)
  Fei-long Zhang (1)
  Zhiyuan Wang (1)
  Li-li Pan (1)
  Ying-ying Shen (1)
  Zhen-zhong Zhang (1)
  
  1. School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, China
  
• ISSN：1572-896X

文摘

The photocytotoxicity of water-dispersed 10000nm fullerene amino acid derivatives nanoparticles was studied. The nanoparticle solution of fullerene derivatives, l-phenylalanine (C60-phe) and glycine (C60-gly), suppressed the in vitro growth of MCF-7 cells lines, induced cancer cells apoptosis, and caused a perturbation of the cell cycle. These nanoparticle solutions increased intracellular reactive oxygen species after irradiation. C60-phe or C60-gly upregulated the expression of phosphorylated (p)p38 mitogen-activated protein kinase (MAPK). N-Acetyl-l-cysteine significantly depressed the composite-induced activation of p38MAPK, and the kinase inhibitor SB203580 significantly prevented C60 derivative-induced cell apoptosis. This study revealed that p38MAPK is activated by C60 nanoparticles through triggering reactive oxygen species generation, leading to cancer cell injuries.