Chaetocin reactivates the lytic replication of Epstein-Barr virus from latency via reactive oxygen species

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• 作者：Shilun Zhang ; Juan Yin ; Jiang Zhong
• 关键词：Epstein ; Barr virus ; reactive oxygen species ; chaetocin
• 刊名：Science China Life Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：60
• 期：1
• 页码：66-71
• 全文大小：
• 刊物主题：Life Sciences, general;
• 出版者：Science China Press
• ISSN：1869-1889
• 卷排序：60

文摘

Oxidative stress, regarded as a negative effect of free radicals in vivo, takes place when organisms suffer from harmful stimuli. Some viruses can induce the release of reactive oxygen species (ROS) in infected cells, which may be closely related with their pathogenicity. In this report, chaetocin, a fungal metabolite reported to have antimicrobial and cytostatic activity, was studied for its effect on the activation of latent Epstein-Barr virus (EBV) in B95-8 cells. We found that chaetocin remarkably up-regulated EBV lytic transcription and DNA replication at a low concentration (50 nmol L−1). The activation of latent EBV was accompanied by an increased cellular ROS level. N-acetyl-L-cysteine (NAC), an ROS inhibitor, suppressed chaetocin-induced EBV activation. Chaetocin had little effect on histone H3K9 methylation, while NAC also significantly reduced H3K9 methylation. These results suggested that chaetocin reactivates latent EBV primarily via ROS pathways.