Gold Nanoparticle (AuNP)-Based Surface-Enhanced Raman Scattering (SERS) Probe of Leukemic Lymphocytes

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• 作者：Qinnan Zhang ; Xiaoxu Lu ; Ping Tang ; Daosen Zhang ; Jindong Tian ; Liyun Zhong
• 关键词：Surface ; enhanced Raman scattering (SERS) ; AuNPs (gold nanoparticles) ; Leukemic lymphocyte ; CD3 ; CD19 ; MBA (4 ; mercaptobenzoic acid)
• 刊名：Plasmonics
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：11
• 期：5
• 页码：1361-1368
• 全文大小：2,454 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Nanotechnology
  Biophysics and Biomedical Physics
  Biochemistry
  
• 出版者：Springer US
• ISSN：1557-1963
• 卷排序：11

文摘

Identification of cellular surface marker is an effective approach in the early diagnosis of tumor. In this study, based on the finite difference time domain (FDTD) simulation, combing the gold nanoparticle (AuNP)-based surface-enhanced Raman scattering (SERS) and the immunolabeling technique, we prepared AuNP-based SERS probe to achieve the surface marker identification of leukemic lymphocytes, in which AuNPs were capped by a Raman reporter of 4-mercaptobenzoic acid (MBA) and antibody, and then encapsulated by polyethylene glycol (PEG). The obtained results showed that the prepared AuNPs/MBA-based SERS probe revealed the higher enhancement factor of Raman signal and the better specific selectivity and targeting function. Using this AuNPs/MBA-based SERS probe, both Jurkat cells and Raji cells can be identified easily. Importantly, this novel AuNPs/MBA-based SERS probe will supply a useful tool in the early diagnosis of leukemic lymphocytes.