Pesticides Residues Rapid Extraction from Panax Ginseng Using a Modified QuEChERS Method for GC–MS

详细信息    查看全文

• 作者：Shuang Liang ; Xuanwei Xu ; Zhongbin Lu
• 关键词：Rapid extraction ; Modified QuEChERS ; GC–MS ; Panax ginseng
• 刊名：Chromatographia
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：78
• 期：23-24
• 页码：1491-1498
• 全文大小：638 KB
• 参考文献：1.Lee NR, Han JS, Kim JS, Choi JE (2011) Korean J Med Crop Sci 19:271-75CrossRef
  2.Yang RJ, Li XW, Yao H, Zhang MCh, Jin YR (2012) Chromatographia 75:281-87CrossRef
  3.Vuksan V, Sievenpiper JL, Koo VYY, Francis T, Beljan ZU, Xu Z, Vidgen E (2001) Arch Intern Med 160:1009-013CrossRef
  4.Lee KG, Jo EK (2012) Food Chem 134:2497-503CrossRef
  5.Zhao C, Hao G, Li HX, Chen LY (2006) Biomed Chromatogr 20:857CrossRef
  6.Hayward DG, Wong JW, Zhang K (2011) J AOAC Int 94:1741CrossRef
  7.Hernández-Borges J, Cabrera JC, Rodríguez-Delgado MA, Hernández-Suarez EM, Saúco VG (2009) Food Chem 113:313-19CrossRef
  8.Garrido FA, Plaza-Bolanos P, Martinez VJL (2008) J Chromatogr A 1203:229-38CrossRef
  9.Oshita D, Jardim ICSF (2014) Chromatographia 77:1291-298CrossRef
  10.Khan IA, Allgood J, Walker LA, Abourashed EA, Schlenk D, Benson WH (2001) J AOAC Int 84:936-39
  11.Hajslova J, Holadova K, Kocourek V (1998) J Chromatogr A 800:283-95CrossRef
  12.Wong JW, Webster MG, Halverson CA, Hengel MJ, Ngim KK, Ebeler SE (2003) J Agr Food Chem 51:1148-461CrossRef
  13.Lehotay SJ, Mastovska K, Lightfield AR, Gates RA (2010) J AOAC Int 93:355-67
  14.Shim JH, Abd EAM, Choi JH, Choi YS (2007) Biomed Chromatogr 21:695-00CrossRef
  15.Anastassiades M, Lehotay SJ, Stajnbaher D, Schenck FJ (2003) J AOAC Int 86:412-31
  
• 作者单位：Shuang Liang (1)
  Xuanwei Xu (2)
  Zhongbin Lu (1)
  
  1. College of Resources and Environment Science, Jilin Agricultural University, Changchun, 130118, Jilin, People’s Republic of China
  2. Ginseng and Antler Products Testing Center of the Ministry of Agricultural PRC, Jilin Agricultural University, Changchun, 130118, Jilin, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Organic Chemistry
  Pharmacy
  Biochemistry
  Plant Sciences
  Measurement Science and Instrumentation
  
• 出版者：Vieweg Verlag
• ISSN：1612-1112

文摘

A modified quick, easy, cheap, effective, rugged and safe (QuEChERS) rapid detection method followed by gas chromatography–tandem mass spectrometry (GC–MS) has been developed for the simultaneous determination of 42 pesticides in Panax ginseng. This method can be different from the other QuEChERS methods in the sense that it uses acetone and n-hexane solution rather than acetonitrile to extract and partition pesticides. This acetone, water and n-hexane solution QuEChERS method consists essentially of two steps: extraction/partitioning and purification. In step 1, P. ginseng was mixed with acetone, water and n-hexane solution, and then partitioned by vortex. In step 2, the top layer (n-hexane) was transferred into a centrifuge tube containing primary secondary amine, activated carbon and C₁₈ for purification. After the centrifuge supernatant was injected into GC–MS. The QuEChERS method was applied in P. ginseng detection and we confirmed that this method can easily extract various types of pesticides from P. ginseng. The rates of recovery for pesticides studied were satisfactory, ranging from 75.3 to 119.4 % for most of the pesticides with a relative standard deviation of less than 13 %. The LOQs ranged between 0.5 and 1.2 μg kg?. The modified QuEChERS method and GC–MS could enable complex pretreatment in P. ginseng analysis quickly and easily. Keywords Rapid extraction Modified QuEChERS GC–MS Panax ginseng