Production of Ultra-high Molecular Weight Poly-γ-Glutamic Acid with Bacillus licheniformis P-104 and Characterization of its Flocculation Properties

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• 作者：Caifeng Zhao (1)
  Yewei Zhang (1)
  Xuetuan Wei (2)
  Zhongbo Hu (1)
  Feiyan Zhu (1)
  Lin Xu (2)
  Mingfang Luo (3) (4)
  Huizhou Liu (2) (5)
  
• 关键词：Bacillus licheniformis ; Fed ; batch fermentation ; Flocculation ; γ ; Polyglutamic acid ; Ultra ; high molecular weight
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：170
• 期：3
• 页码：562-572
• 全文大小：266KB
• 参考文献：1. Salehizadeh, H., & Shojaosadati, S. A. (2001). / Biotechnology Advances, 19, 371-85. CrossRef
  2. Xiong, Y. Y., Wang, Y. P., Yu, Y., Li, Q. B., Wang, H. T., Chen, R. H., et al. (2010). / Applied and Environmental Microbiology, 76, 2778-782. CrossRef
  3. Salehizadeh, H., Vossoughi, M., & Alemzadeh, I. (2000). / Biochemical Engineering Journal, 5, 39-4. CrossRef
  4. Bajaj, I. B., & Singhal, R. S. (2011). / Food and Bioprocess Technology, 4, 745-52. CrossRef
  5. Shih, I. L., Van, Y. T., Yeh, L. C., Lin, H. G., & Chang, Y. N. (2001). / Bioresource Technology, 78, 267-72. CrossRef
  6. Buescher, J. M., & Margaritis, A. (2007). / Critical Reviews in Biotechnology, 27, 1-9. CrossRef
  7. Kubota, H., Nambu, Y., & Endo, T. (1996). / Journal of Polymer Science Part A: Polymer Chemistry, 34, 1347-351. CrossRef
  8. Taniguchi, M., Kato, K., Matsui, O., Ping, X., Nakayama, H., Usuki, Y., et al. (2005). / Journal of Bioscience and Bioengineering, 100, 207-11. CrossRef
  9. Taniguchi, M., Kato, K., Shimauchi, A., Ping, X., Nakayama, H., Fujita, K.-I., et al. (2005). / Journal of Bioscience and Bioengineering, 99, 245-51. CrossRef
  10. Taniguchi, M., Kato, K., Shimauchi, A., Xu, P., Fujita, K., Tanaka, T., et al. (2005). / Journal of Bioscience and Bioengineering, 99, 130-35. CrossRef
  11. Soliman, N. A., Berekaa, M. M., & Abdel-Fattah, Y. R. (2005). / Applied Microbiology and Biotechnology, 69, 259-67. CrossRef
  12. Li, X. Z., Zhang, D. H., Chen, F., Ma, J., Dong, Y. H., & Zhang, L. H. (2004). / International Journal of Systematic and Evolutionary Microbiology, 54, 2131-136. CrossRef
  13. Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). / Molecular Biology and Evolution, 24, 1596-599. CrossRef
  14. Do, J. H., Chang, H. N., & Lee, S. Y. (2001). / Biotechnology and Bioengineering, 76, 219-23. CrossRef
  15. Yokoi, H., Natsuda, O., Hirose, J., Hayashi, S., & Takasaki, Y. (1995). / Journal of Fermentation and Bioengineering, 79, 378-80. CrossRef
  16. Wu, J. Y., & Ye, H. F. (2007). / Process Biochemistry, 42, 1114-123. CrossRef
  17. Ho, G. H., Yang, T. H., & Yang, J. (2009). United States: Tung Hai Biotechnology Corporation.
  18. Hezayen, F. F., Rehm, B. H. A., Eberhardt, R., & Steinbuchel, A. (2000). / Applied Microbiology and Biotechnology, 54, 319-25. CrossRef
  19. Shimizu, K., Nakamura, H., & Ashiuchi, M. (2007). / Applied and Environmental Microbiology, 73, 2378-379. CrossRef
  20. Ezeji, T. C., Qureshi, N., & Blaschek, H. P. (2005). / Journal of Biotechnology, 115, 179-87. CrossRef
  21. Ho, G. H., Ho, T. I., Hsieh, K. H., Su, Y. C., Lin, P. Y., Yang, J., et al. (2006). / Journal of the Chinese Chemistry Society, 53, 1363-384.
  22. Cromwick, A. M., Birrer, G. A., & Gross, R. A. (1996). / Biotechnology and Bioengineering, 50, 222-27. CrossRef
  23. Bajaj, I. B., & Singhal, R. S. (2010). / Biotechnology and Bioprocess Engineering, 15, 635-40. CrossRef
  24. Bajaj, I. B., & Singhal, R. S. (2009). / Food Technology and Biotechnology, 47, 313-22.
  25. Yang, Z. H., Huang, J., Zeng, G. M., Ruan, M., Zhou, C. S., Li, L., et al. (2009). / Bioresource Technology, 100, 4233-239. CrossRef
  26. Watanabe, T., Ina, T., Ogawa, K., Matsumoto, T., Sawa, S., & Ono, S. (1970). / Bulletin of the Chemical Society of Japan, 43, 3939-940. CrossRef
  27. Besra, L., Sengupta, D. K., Roy, S. K., & Ay, P. (2004). / Separation and Purification Technology, 37, 231-46. CrossRef
  28. Yokoi, H., Arima, T., Hirose, J., Hayashi, S., & Takasaki, Y. (1996). / Journal of Fermentation and Bioengineering, 82, 84-7. CrossRef
  29. Hsu, J. P., & Lin, D. P. (1996). / Colloid & Polymer Science, 274, 172-77. CrossRef
  
• 作者单位：Caifeng Zhao (1)
  Yewei Zhang (1)
  Xuetuan Wei (2)
  Zhongbo Hu (1)
  Feiyan Zhu (1)
  Lin Xu (2)
  Mingfang Luo (3) (4)
  Huizhou Liu (2) (5)
  
  1. College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China
  2. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China
  3. Technology Transfer Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China
  4. West Beichen Road, Chaoyang District, Beijing, 100101, China
  5. No. 1 Bei Er Tiao, Zhong Guan Cun, Haidian District, Beijing, 100190, China
  
• ISSN：1559-0291

文摘

A novel strain of Bacillus licheniformis P-104 was isolated from Chinese soybean paste to produce a bioflocculant. The bioflocculant was confirmed as ultra-high molecular weight poly-γ-glutamic acid (γ-PGA) using Fourier transform infrared spectrum, high-performance liquid chromatography, and gel permeation chromatography with multi-angle laser light scattering. The production technology and flocculation properties of γ-PGA were investigated. By fed-batch fermentation in a 7-L bioreactor, the maximum γ-PGA yield reached 41.6?g?L? with a productivity rate of 1.07?g?L??h?. The flocculating activity of γ-PGA for kaolin suspension was 33.5?±-.6 1/OD under the optimized flocculation conditions (6?mM Ca2+, 1.5?mg?L? γ-PGA, and pH?6.0). The optimized dosage of γ-PGA for flocculation was just about 30?% of that of reported γ-PGA produced by other strains. Moreover, the flocculation activity of γ-PGA produced by strain P-104 was much higher than commercial γ-PGA with the molecular weight ranging 200-00?kDa and 1,500-,500?kDa. This study provided a promising strain and an efficient method for production of ultra-high molecular weight γ-PGA which could be used as a potential green bioflocculant.