Cloning and Characterization of a Novel KRAB-domain-containing Zinc Finger Gene (ZNF284L)^†

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• 作者：Gang Yin ; Chaoneng Ji ; Li Zeng ; Zebin Wang ; Jiayan Wang ; Zhouliang Shen ; Tong Wu ; Shaohua Gu ; Yi Xie and Yumin Mao
• 关键词：C2H2 zinc finger motif ; expression pattern ; KRAB domain ; ZNF284L
• 刊名：Molecular Biology Reports
• 出版年：2006
• 出版时间：June 2006
• 年：2006
• 卷：33
• 期：2
• 页码：137-144
• 全文大小：548 KB

文摘

The zinc finger gene (ZNF) family plays an important role in the regulation of transcription. This study reports the cloning and characterization of a novel human zinc finger protein cDNA (ZNF284L) from fetal brain cDNA library. The ZNF284L cDNA is 2223 bp in length encoding a 593-aa polypeptide. The protein contains a KRAB A+b box and eleven C2H2 type zinc finger motifs. ZNF284L gene is mapped to 19q13.2–19q13.3 with 5 exons, and the expression pattern of ZNF284L gene was also examined by reverse transcription polymerase chain reaction (RT-PCR). The transcripts were detected in the human lung, liver, pancreas, thymus, heart, placenta, spleen, prostate, ovary, small intestine and colon, but in human brain, skeletal muscle, kidney, testis and peripheral blood leukocyte, no expression was detected.