Highly sensitive enzymatic determination of urea based on the pH-dependence of the fluorescence of graphene quantum dots

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• 作者：Taili Shao ; Ping Zhang ; Lin Tang ; Shujuan Zhuo ; Changqing Zhu
• 关键词：Graphene quantum dots ; Urea ; Detection ; Urease
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：182
• 期：7-8
• 页码：1431-1437
• 全文大小：4,086 KB
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• 作者单位：Taili Shao (1) (2)
  Ping Zhang (1)
  Lin Tang (2)
  Shujuan Zhuo (1)
  Changqing Zhu (1)
  
  1. Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China
  2. School of Pharmacy, Wannan Medical College, Wuhu, 241002, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

We report on a nanoparticle-based fluorescent sensing scheme for urea. It is based on the finding that graphene quantum dots (GQDs) display pH-sensitive green fluorescence if photoexcited at 460?nm. Fluorescence is gradually quenched due to an increase in the local pH value as a result of the hydrolysis of urea as catalyzed by urease. The effect was used to quantify urea in the 0.1-00?mM concentration range, with a limit of detection of 0.01?mM. The method was successfully applied to the determination of urea in human serum samples. The method is simple, effective, and therefore holds promise as a platform for sensing urea in blood.