The Role of Endogenous Ethylene in Carbohydrate Metabolism of Medicago sativa L. Somatic Embryos in Relation to Their Regenerative Ability
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- 作者：Ewa K?pczyńska (1)
Sylwia Zielińska (1) - 关键词：Alfalfa ; α ; amylase ; Ethylene biosynthesis inhibitors ; Medicago sativa L. ; Soluble carbohydrates ; Somatic embryo germination and conversion ; Starch
- 刊名：Journal of Plant Growth Regulation
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：32
- 期：1
- 页码：191-199
- 全文大小：241KB
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Sylwia Zielińska (1)
1. Department of Plant Biotechnology, Faculty of Biology, University of Szczecin, W?ska 13, 71-415, Szczecin, Poland - ISSN：1435-8107
文摘
Effects of the ethylene biosynthesis inhibitors salicylic acid (SA) and aminoethoxyvinylglycine (AVG) on germination of Medicago sativa L. somatic embryos and their conversion to seedlings in relation to carbohydrate content and α-amylase activity were studied. Both SA, an inhibitor of ACC oxidase, and AVG, an inhibitor of ACC synthase, when present in the regeneration medium (0.1 and 1?μM) were found to drastically reduce the embryo germination rate. In addition, SA and AVG were found to almost completely or completely, respectively, arrest the process of embryo conversion to seedlings. The inhibitory effects of SA and AVG on germination and conversion may indicate that the processes required endogenous ethylene. AVG and SA clearly slowed down starch disappearance during the 48-h imbibition in the regeneration medium prior to radicle elongation, which was correlated with inhibition of the activity of α-amylase, an enzyme responsible for starch hydrolysis. It is probable that ethylene may activate α-amylase in the germinating alfalfa somatic embryos. In contrast, the disappearance of soluble sugars in the embryos in the presence of the inhibitors tested was accelerated. The disappearance of soluble sugars (to null or almost null) in embryos was faster in the presence of SA in the regeneration medium after 24 and 48?h compared to the disappearance rate with AVG present in the medium. Only glucose was present after a 48-h incubation in the regeneration medium in the presence of the two ethylene biosynthesis inhibitors, in contrast to the control embryos in which glucose was not detected.