Culture observation and molecular phylogenetic analysis on the blooming green alga Chaetomorpha valida (Cladophorales, Chlorophyta) from China

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• 作者：Yunyan Deng (12216) (22216)
  Xiaorong Tang (32216)
  Zifeng Zhan (12216)
  Linhong Teng (12216) (22216)
  Lanping Ding (12216) (42216)
  Bingxin Huang (12216) (42216)
  
• 关键词：blooming green algae ; Chaetomorpha valida ; branch ; culture observation ; heteromorphic life cycle ; molecular phylogeny
• 刊名：Chinese Journal of Oceanology and Limnology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：31
• 期：3
• 页码：552-559
• 全文大小：671KB
• 参考文献：1. Altschul S, Gish W, Miller W, Myers E, Lipman D. 1990. A basic local alignment search tool. / J. Mol. Biol., 215: 403-10.
  2. Bakker F T, Olsen J L, Stam W T. 1995a. Evolution of nuclear rDNA ITS sequences in the / Cladophora albida / / sericea clade (Chlorophyta). / J. Mol. Evol., 40: 640-51. CrossRef
  3. Bakker F T, Olsen J L, Stam W T. 1995b. Global phylogeography in the cosmopolitan species / Cladophora vagabunda (Chlorophyta) based on nuclear rDNA internal transcribed spacer sequences. / Eur. J. Phycol., 30: 197-08. CrossRef
  4. Blomster J, Maggs C A, Stanhope M J. 1999. Extensive intraspecific morphological variation in / Enteromorpha muscoides (Chlorophyta) revealed by molecular analysis. / J. Phycol., 35: 575-86. CrossRef
  5. Chi Y X, Wang L M, Luan R X, Wang H W. 2009. / Chaetomorpha valida, a new recorded green alga species in genus / Chaetomorpha Kützing in China. / Fisheries Science, 28(3): 162-63. (in Chinese with English abstract)
  6. Deng Y Y, Tang X R, Huang B X, Ding L P. 2011a. Life history of / Chaetomorpha valida (Cladophoraceae, Chlorophyta) in culture. / Bot. Mar., 54(6): 551-56. CrossRef
  7. Deng Y Y, Tang X R, Huang B X, Ding L P. 2011b. The temperature character of marine green alga, / Chaetomorpha valida, with analysis of its diffusion potential in marine algal flora of China. / Oceanologia et Limnologia Sinica, 42(3): 404-08. (in Chinese with English summary)
  8. Deng Y Y, Tang X R, Huang B X, Ding L P. 2012a. Effect of temperature and irradiance on the growth and reproduction of the green macroalga, / Chaetomorpha valida (Cladophoraceae, Chlorophyta). / J. Appl. Phycol., 24(4): 927-33. CrossRef
  9. Deng Y Y, Tang X R, Huang B X, Teng L H, Ding L P. 2012b. Molecular identification and culture observation on / Acrochaete leptochaete (Chaetophoraceae, Chlorophyta) from China. / Chin. J. Oceanol. Limnol., 30(3): 476-84. CrossRef
  10. Ding L P, Luan R X. 2013. Flora Algarum Marinarum Sinicarum Tomus IV. Chlorophyta No. I. Science Press, Beijing, China. (in Chinese)
  11. Guindon S, Gascuel O. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. / Syst. Biol., 52: 696-04. CrossRef
  12. Guiry M D, Guiry G M. 2012. AlgaeBase. World-Wide Electronic Publication, National University of Ireland, Galway. http://www.algaebase.org. Accessed on 2012-03-28.
  13. Hall T A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. / Nucleic Acids Symposium Series, 41: 95-8.
  14. Hanyuda T, Wakana I, Arai S, Miyaji K, Watano Y, Ueda K. 2002. Phylogenetic relationships within Ladophorales (Ulvophyceae, Chlorophyta) inferred from 18S RNA gene sequences, with special reference to / Aegagropila linnaei. / J. Phycol., 38: 564-71. CrossRef
  15. Hayden H S, Waaland J R. 2002. Phylogenetic systematic of the / Ulvaceae (Ulvales, Ulvophyceae) using chloroplast and nuclear DNA sequence. / Phycologia, 38: 1 200- 212.
  16. Hayden H S, Blomster J, Maggs C A, Silva P C, Stanhope M J, Waaland J R. 2003. Linnaeus was right all along: / Ulva and / Enteromorpha are not distinct genera. / Eur. J. Phycol., 38: 277-94. CrossRef
  17. Kornmann P. 1972. Ein beitrag zur taxonomie der gattung / Chaetomorpha (Cladophorales, Chlorophyta). / Helgol. Wiss. Meeresunters., 23: 1-1. CrossRef
  18. Leliaert F, De Clerck O, Verbruggen H, Boedeker C, Coppejans E. 2007. Molecular phylogeny of the Siphonocladales (Chlorophyta: Cladophorophyceae). / Mol. Phylogenet. Evol., 44: 1 237- 256. CrossRef
  19. Leliaert F, D’hondt S, Tyberghein L, Verbruggen H, De Clerck O. 2011. Atypical development of / Chaetomorpha antennina in culture (Cladophorales, Chlorophyta). / Phycol. Res., 59: 91-7. CrossRef
  20. Leliaert F, Verbruggen H, Wysor B, De Clerck O. 2009. DNA taxonomy in morphologically plastic taxa: algorithmic species delimitation in the / Boodlea complex (Chlorophyta: Cladophorales). / Mol. Phylogenet. Evol., 53: 122-33. CrossRef
  21. Mathieson A C, Norton T A, Neushul M. 1981. The taxonomic implications of genetic and environmentally induced variations in seaweed morphology. / Bot. Rev., 47: 313-47. CrossRef
  22. Nienhuis P H. 1975. Biosystematics and Ecology of / Rhizoclonium riparium (Roth) Harv. (Chlorophyceae: Cladophorales) in the Estuarine Area of the Rivers Rhine, Meuse and Scheldt. Booklet Bronder Offset BV, Rotterdam, Netherlands. p.6-8.
  23. Patel R J. 1971. Growth of members of Cladophorales in experimental culture. / Phykos, 10: 40-3.
  24. Posada D, Crandall K A. 1998. Model test: testing the model of DNA substitution. / Bioinformatics, 14: 817-18. CrossRef
  25. Price W M. 1967. Some Aspects of the Biology and Taxonomy of the Unbranched Cladophorales in the British Islands. Dissertation, University of Liverpool, England. 107p.
  26. Ronquist F, Huelsenbeck J P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. / Bioinformatics, 19: 1 572- 574. CrossRef
  27. South G R, Skelton P A. 2003. Catalogue of the marine benthic macroalgae of the Fiji Islands, South Pacific. / Aust. Syst. Bot., 16: 699-58. CrossRef
  28. Teng L H. 2011. Study on Morphology and Molecular Phylogeny of Cladophorales (Chlorophyta) along China Sea Coast, with Its DNA Barcoding Based on ITS and 18S rDNA Sequences. Master Dissertation, Graduate University of Chinese Academy of Sciences, Beijing, China. 121p. (in Chinese with English abstract)
  29. Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. 1997. The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. / Nucleic. Acids. Res., 25: 4 876- 882. CrossRef
  30. Van den Hoek C. 1963. Revision of the European Species of / Cladophora. Leide: E. J. Brill. 248p.
  31. Womersley H B S. 1984. The Marine Benthic Flora of Southern Australia. Government Printer, Adelaide, South Australia. 329p.
  32. Yoshida T. 1998. Marine Algae of Japan. Uchida Rokakuho Publishing, Tokyo, Japan. 1222p. (in Japanese)
  
• 作者单位：Yunyan Deng (12216) (22216)
  Xiaorong Tang (32216)
  Zifeng Zhan (12216)
  Linhong Teng (12216) (22216)
  Lanping Ding (12216) (42216)
  Bingxin Huang (12216) (42216)
  
  12216. Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
  22216. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China
  32216. Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao, 266003, China
  42216. Shantou University, Shantou, 515063, China
  
• ISSN：1993-5005

文摘

The marine green alga Chaetomorpha valida fouls aquaculture ponds along the coastal cities of Dalian and Rongcheng, China. Unialgal cultures were observed under a microscope to determine the developmental morphological characters of C. valida. Results reveal that gametophytic filaments often produce lateral branches under laboratory culture conditions, suggesting an atypical heteromorphic life cycle of C. valida between unbranched sporophytes and branched gametophytes, which differs from typical isomorphic alternation of Chaetomorpha species. The shape of the basal attachment cell, an important taxonomic character within the genus, was found variable depending on environmental conditions. The 18S rDNA and 28S rDNA regions were used to explore the phylogenetic affinity of the taxa. Inferred trees from 18S rDNA sequences revealed a close relationship between C. valida and Chaetomorpha moniligera. These results would enrich information in general biology and morphological plasticity of C. valida and provided a basis for future identification of green tide forming algae.