Poly(γ-glutamic acid) enhanced tolerance to salt stress by promoting proline accumulation in Brassica napus L.

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• 作者：Peng Lei ; Zongqi Xu ; Jinfeng Liang ; Xiaohui Luo ; Yunxia Zhang…
• 关键词：γ ; PGA ; Salt stress ; K+/Na+ ratio ; Antioxidase ; Proline accumulation ; Proline metabolism regulation genes
• 刊名：Plant Growth Regulation
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：78
• 期：2
• 页码：233-241
• 全文大小：617 KB
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• 作者单位：Peng Lei (1) (2)
  Zongqi Xu (1) (2)
  Jinfeng Liang (2)
  Xiaohui Luo (3)
  Yunxia Zhang (1) (2)
  Xiaohai Feng (1) (2)
  Hong Xu (1) (2)
  
  1. State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing, 211816, China
  2. College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211816, China
  3. Maanshan Soil and Fertilizer Station, Maanshan, 243000, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

Poly(γ-glutamic acid) (γ-PGA) is a new plant growth regulator with extensive application prospects. The effects of γ-PGA on rape seedlings under salt stress and the mechanism of action were investigated via hydroponic experiments. A salinity model was simulated by exposing the roots of rape seedlings to 100 mM NaCl solution for 48, 96 and 144 h. We determined that the growth of rape seedlings treated with NaCl was significantly inhibited. However, after the application of γ-PGA under NaCl stress, the dry weights of the entire plant, the shoot and the root were increased by 37.4, 38.8 and 34.1 %, respectively, at 144 h compared with those of the NaCl group. Moreover, the K+/Na+ ratio, proline content and antioxidant enzyme activity were all evidently enhanced, and the malondialdehyde content was significantly reduced in samples treated with γ-PGA. Proline metabolism regulation genes, including pyrroline-5-carboxylate synthetase genes (BnP5CS1 and BnP5CS2) and proline dehydrogenase gene (BnPDH), were also upregulated and downregulated by γ-PGA, respectively. Results showed that γ-PGA improved resistance to salt stress in rape seedlings by activating the proline synthesis pathway and promoting proline accumulation.