Direct determination of trace refractory elements in human serum by ETV–ICP–MS with in-situ matrix removal

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• 作者：Shengqing Li ; Bin Hu ; Zucheng Jiang and Rui Chen
• 关键词：ETV–ICP–MS ; Refractory elements ; Serum ; In ; situ matrix removal ; Poly(tetrafluoroethylene)
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2004
• 出版时间：August 2004
• 年：2004
• 卷：379
• 期：7-8
• 页码：1076-1082
• 全文大小：256 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

A method for in-situ removal of matrix is proposed for direct determination of trace refractory elements in human serum by ETV–ICP–MS with the use of poly(tetrafluoroethylene) (PTFE) as fluorinating reagent. Attention has been paid to investigating the vaporization behavior both of refractory elements of interest and of matrix elements (Na, K, Ca, Mg, Cl, S, and P) in a graphite furnace with the PTFE modifier present or not. It was shown that potential interferences from the organic and inorganic matrices in the serum sample could be eliminated or reduced to a negligible level by appropriate dilution of the serum and deliberate optimization of the ETV temperature program. The proposed method has been applied to the direct simultaneous determination of V, Cr, Mo, Ba, La, Ce, and W in human serum. The limits of detection for fivefold diluted serum were 0.18 (V), 0.229 (Cr), 0.050 (Mo), 0.328 (Ba), 0.031 (La), 0.038 (Ce), and 0.019 ng mL–1 (W), respectively, and the relative standard deviations of the method were in the range 4–15% (2 ng mL–1 in serum, n=3).