文摘
A novel bacterium, designated U33T, was isolated from a soil sample collected in Mykhailyky, Poltavs’ka oblast, Ukraine. The bacterium was aerobic, Gram-positive, spore-forming, and consists of motile rods. The taxonomic position of strain U33T was studied by a polyphasic approach, and the results clearly showed that the phenotypic and chemotaxonomic properties are consistent with those of the genus Aneurinibacillus. The phylogenic analysis with 16S rRNA gene sequence of strains U33T showed the highest sequence similarity to those of Aneurinibacillus aneuriniticus ATCC 12856T (96.7 %), Aneurinibacillus migulanus DSM 2895T (96.7 %), Aneurinibacillus danicus NCIMB 13288T (95.8 %), and lower sequence similarity with other members of the genus Aneurinibacillus. Growth was observed at 20–55 °C (optimum, 37 °C) at pH 5.0–9.0 (optimum, pH 7) and with 0–5 % (w/v) NaCl (optimum, 2 % NaCl). The predominant menaquinone was MK-7 and the cell wall peptidoglycan consist of meso-diaminopimelic acid. The major cellular fatty acids are iso-C15:0 (58.0 %) and anteiso-C15:0 (13.2 %). The DNA G+C content of the strain U33T was 45.8 %. The physiological and chemotaxonomic characteristics distinguish strain U33T from the validly published species of genus Aneurinibacillus, and therefore, we consider this strain to represent a novel species of the genus Aneurinibacillus. The name Aneurinibaciilus humi sp. nov. is proposed with strain U33T (= KEMC7305-119T = JCM19865T) as the type strain.