Enrichment of cardiac differentiation by a large starting number of embryonic stem cells in embryoid bodies is mediated by the Wnt11-JNK pathway

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• 作者：Ming Chen (1)
  Cheng Qian (1)
  Lin-Lin Bi (2)
  Fang Zhao (1)
  Guang-Yu Zhang (1)
  Zhi-Quan Wang (1)
  Xue-Dong Gan (1)
  Yang-Gan Wang (1)
  
  1. Department of Cardiology ; Zhongnan Hospital of Wuhan University ; Wuhan ; 430071 ; China
  2. Key Laboratory of Neurological Disease ; Ministry of Education ; Huazhong University of Science and Technology and Tongji Medical College ; Wuhan ; 430030 ; China
  
• 关键词：Cardiomyocyte ; Embryonic stem cells ; Hanging drop ; Jun N ; terminal kinase ; Wnt11
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：37
• 期：2
• 页码：475-481
• 全文大小：697 KB
• 参考文献：1. Boheler KR, Czyz J, Tweedie D, Yang HT, Anisimov SV, Wobus AM (2002) Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res 91:189鈥?01 CrossRef
  2. Chen K, Wu L, Wang ZZ (2008) Extrinsic regulation of cardiomyocyte differentiation of embryonic stem cells. J Cell Biochem 104:119鈥?28 CrossRef
  3. Chen M, Lin YQ, Xie SL, Wang JF (2010) Mitogen-activated protein kinase in endothelin-1-induced cardiac differentiation of mouse embryonic stem cells. J Cell Biochem 111:1619鈥?628 CrossRef
  4. Chen M, Lin YQ, Xie SL, Wu HF, Wang JF (2011) Enrichment of cardiac differentiation of mouse embryonic stem cells by optimizing the hanging drop method. Biotechnol Lett 33:853鈥?58 CrossRef
  5. Chen M, Bi LL, Wang ZQ, Zhao F, Gan XD, Wang YG (2013) Time-dependent regulation of neuregulin-1beta/ErbB/ERK pathways in cardiac differentiation of mouse embryonic stem cells. Mol Cell Biochem 380:67鈥?2 CrossRef
  6. Cohen ED, Miller MF, Wang Z, Moon RT, Morrisey EE (2012) Wnt5a and Wnt11 are essential for second heart field progenitor development. Development 139:1931鈥?940 CrossRef
  7. Eisenberg CA, Gourdie RG, Eisenberg LM (1997) Wnt-11 is expressed in early avian mesoderm and required for the differentiation of the quail mesoderm cell line QCE-6. Development 124:525鈥?36
  8. Flaherty MP, Abdel-Latif A, Li Q, Hunt G, Ranjan S, Ou Q, Tang XL, Johnson RK, Bolli R, Dawn B (2008) Noncanonical Wnt11 signaling is sufficient to induce cardiomyogenic differentiation in unfractionated bone marrow mononuclear cells. Circulation 117:2241鈥?252 CrossRef
  9. Murry CE, Keller G (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132:661鈥?80 CrossRef
  10. Pandur P, Lasche M, Eisenberg LM, Kuhl M (2002) Wnt-11 activation of a non-canonical Wnt signalling pathway is required for cardiogenesis. Nature 418:636鈥?41 CrossRef
  11. Park J, Cho CH, Parashurama N, Li Y, Berthiaume F, Toner M, Tilles AW, Yarmush ML (2007) Microfabrication-based modulation of embryonic stem cell differentiation. Lab Chip 7:1018鈥?028 CrossRef
  12. Rungarunlert S, Klincumhom N, Bock I, Nemes C, Techakumphu M, Pirity MK, Dinnyes A (2011) Enhanced cardiac differentiation of mouse embryonic stem cells by use of the slow-turning, lateral vessel (STLV) bioreactor. Biotechnol Lett 33:1565鈥?573 CrossRef
  13. Schroeder KE, Condic ML, Eisenberg LM, Yost HJ (1999) Spatially regulated translation in embryos: asymmetric expression of maternal Wnt-11 along the dorsal鈥搗entral axis in / Xenopus. Dev Biol 214:288鈥?97 CrossRef
  14. Schukur L, Zorlutuna P, Cha JM, Bae H, Khademhosseini A (2013) Directed differentiation of size-controlled embryoid bodies towards endothelial and cardiac lineages in RGD-modified poly(ethylene glycol) hydrogels. Adv Healthc Mater 2:195鈥?05 CrossRef
  15. Xiang G, Yang Q, Wang B, Sekiya N, Mu X, Tang Y, Chen CW, Okada M, Cummins J, Gharaibeh B, Huard J (2011) Lentivirus-mediated Wnt11 gene transfer enhances cardiomyogenic differentiation of skeletal muscle-derived stem cells. Mol Ther 19:790鈥?96 CrossRef
  16. Yamaguchi TP, Bradley A, McMahon AP, Jones S (1999) A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo. Development 126:1211鈥?223
  17. Yang DH, Yoon JY, Lee SH, Bryja V, Andersson ER, Arenas E, Kwon YG, Choi KY (2009) Wnt5a is required for endothelial differentiation of embryonic stem cells and vascularization via pathways involving both Wnt/beta-catenin and protein kinase Calpha. Circ Res 104:372鈥?79 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Embryoid bodies (EBs) with large starting numbers of embryonic stem cells (ESCs) have a greater degree of cardiac differentiation than from low numbers of EBs. However, the biological roles of signaling molecules in these effects are not well understood. Here, we show that groups of EBs with different starting numbers of ESCs had differential gene expression patterns for Wnt5a and Wnt11. Wnt11 significantly increased the percentage of beating EBs by up-regulating the expression of the cardiac-specific genes. Wnt5a did not show these effects. Moreover, Wnt11 significantly increased the level of phosphorylated Jun N-terminal kinase. The inhibition of the JNK pathway by SP600125 blocked the effects of Wnt11. Thus, enrichment of cardiac differentiation in groups of EBs with a larger starting number of ESCs is mediated by the Wnt11-JNK pathway.