Analysis of Cancer Marker in Tissues with Hadamard Transform Fluorescence Spectral Microscopic Imaging

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• 作者：Hao Xu (1) (2)
  Chuang Chen (3)
  Yue He (1)
  Hong-Wu Tang (1)
  Zhi-Ling Zhang (1)
  Yan Li (3)
  Dai-Wen Pang (1)
  
  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education) ; College of Chemistry and Molecular Sciences ; Wuhan University ; Wuhan ; 430072 ; China
  2. Cold and Arid Regions Environmental and Engineering Research Institute (CAREERI) of the Chinese Academy of Sciences (CAS) ; Lanzhou ; 730000 ; China
  3. Department of Oncology ; Zhongnan Hospital of Wuhan University & Hubei Key Laboratory of Tumor Biological Behaviors ; Wuhan ; 430071 ; China
  
• 关键词：Cancer biomarkers ; Estrogen receptor ; Quantum dots ; Fluorescence imaging ; Spectral imaging ; Hadamard transform
• 刊名：Journal of Fluorescence
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：25
• 期：2
• 页码：397-402
• 全文大小：687 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Biomedicine
  Biophysics and Biomedical Physics
  Biotechnology
  Biochemistry
  Analytical Chemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-4994

文摘

Quantum dots (QDs) probes were used to tag and trace cancer biomarkers in cancer tissues based on the system of home-made Hadamard transform (HT) spectral microscopic imaging, which can be applied to provide high-resolution fluorescence spectrum and image of single cells and tissues. In situ fluorescence imaging for cancer marker proteins, such as estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), proliferating cell nuclear antigen (PCNA) and cytokeratin 20 (CK20) in tumor tissues, were realized by using the HT system to capture quantitative information for these proteins when tumor tissues were immunostained with QDs probes. A method to evaluate tumor malignancy of the specimens based on in situ analysis of distribution of marker proteins was proposed based on the comparative study of positive samples and negative controls. The investigation of ER contents of the cores in breast cancer tissue microarrays (TMAs) shows that the technique of QDs-immunohistochemistry (IHC)/HT spectral imaging is more sensitive than conventional IHC method. The results also demonstrate that the QDs-IHC/HT spectral imaging technique can be applied to visualize and quantitatively measure the subcellular molecules inside tumor tissues, and the coupling of HT spectral imaging to the probing of subcellular molecules with QDs has great potential in biology and medical diagnosis.