Identification and comparative analysis of differentially expressed miRNAs in leaves of two wheat (Triticum aestivum L.) genotypes during dehydration stress

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• 作者：Xingli Ma (1) (2) (3)
  Zeyu Xin (1) (2) (3)
  Zhiqiang Wang (1) (2) (3)
  Qinghua Yang (1) (2) (3)
  Shulei Guo (1) (2) (3)
  Xiaoyang Guo (1) (2) (3)
  Liru Cao (1) (2) (3)
  Tongbao Lin (1) (2) (3)
  
  1. College of Agronomy ; Henan Agricultural University ; Zhengzhou ; 450002 ; China
  2. Collaborative Innovation Center of Henan Grain Crops ; Zhengzhou ; 450002 ; China
  3. National Key Laboratory of Wheat and Maize Crop Science ; Zhengzhou ; 450002 ; China
  
• 关键词：Dehydration stress ; Triticum aestivum L ; Differentially expressed miRNAs ; Comparative analysis
• 刊名：BMC Plant Biology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：15
• 期：1
• 全文大小：1,623 KB
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• 刊物主题：Plant Sciences; Agriculture; Tree Biology;
• 出版者：BioMed Central
• ISSN：1471-2229

文摘

Background MicroRNAs (miRNAs) play critical roles in the processes of plant growth and development, but little is known of their functions during dehydration stress in wheat. Moreover, the mechanisms by which miRNAs confer different levels of dehydration stress tolerance in different wheat genotypes are unclear. Results We examined miRNA expressions in two different wheat genotypes, Hanxuan10, which is drought-tolerant, and Zhengyin1, which is drought-susceptible. Using a deep-sequencing method, we identified 367 differentially expressed miRNAs (including 46 conserved miRNAs and 321 novel miRNAs) and compared their expression levels in the two genotypes. Among them, 233 miRNAs were upregulated and 10 were downregulated in both wheat genotypes after dehydration stress. Interestingly, 13 miRNAs exhibited opposite patterns of expression in the two wheat genotypes, downregulation in the drought-tolerant cultivar and upregulation in the drought-susceptible cultivar. We also identified 111 miRNAs that were expressed predominantly in only one or the other genotype after dehydration stress. We verified the expression patterns of a number of representative miRNAs using qPCR analysis and northern blot, which produced results consistent with those of the deep-sequencing method. Moreover, monitoring the expression levels of 10 target genes by qPCR analysis revealed negative correlations with the levels of their corresponding miRNAs. Conclusions These results indicate that differentially expressed patterns of miRNAs between these two genotypes may play important roles in dehydration stress tolerance in wheat and may be a key factor in determining the levels of stress tolerance in different wheat genotypes.