Size and shape characterization of hydrated and desiccated exosomes

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• 作者：Vasiliy S. Chernyshev (1)
  Rakesh Rachamadugu (2)
  Yen Hsun Tseng (1)
  David M. Belnap (3)
  Yunlu Jia (1)
  Kyle J. Branch (1)
  Anthony E. Butterfield (1)
  Leonard F. Pease III (1) (4) (5)
  Philip S. Bernard (2) (6)
  Mikhail Skliar (1)
  
  1. Department of Chemical Engineering ; University of Utah ; 50 Central Campus Drive ; Salt Lake City ; UT ; 84112 ; USA
  2. Huntsman Cancer Institute ; University of Utah ; University Medical Center ; 2000 Circle of Hope Drive ; Salt Lake City ; UT ; 84112 ; USA
  3. Department of Biochemistry and Structural Biology ; University of Utah ; 257S 1400 E ; Salt Lake City ; UT ; 84112 ; USA
  4. Division of Gastroenterology ; Hepatology ; and Nutrition ; Department of Internal Medicine ; University of Utah ; Salt Lake City ; UT ; 84112 ; USA
  5. Department of Pharmaceutics & Pharmaceutical Chemistry ; University of Utah ; Salt Lake City ; UT ; 84112 ; USA
  6. Department of Pathology ; University of Utah ; North Medical Drive East ; Salt Lake City ; UT ; 84112 ; USA
  
• 关键词：Hydrated and desiccated exosomes ; Size and shape characterization
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：407
• 期：12
• 页码：3285-3301
• 全文大小：1,743 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

Exosomes are stable nanovesicles secreted by cells into the circulation. Their reported sizes differ substantially, which likely reflects the difference in the isolation techniques used, the cells that secreted them, and the methods used in their characterization. We analyzed the influence of the last factor on the measured sizes and shapes of hydrated and desiccated exosomes isolated from the serum of a pancreatic cancer patient and a healthy control. We found that hydrated exosomes are close-to-spherical nanoparticles with a hydrodynamic radius that is substantially larger than the geometric size. For desiccated exosomes, we found that the desiccated shape and sizing are influenced by the manner in which drying occurred. Isotropic desiccation in aerosol preserves the near-spherical shape of the exosomes, whereas drying on a surface likely distorts their shapes and influences the sizing results obtained by techniques that require surface fixation prior to analysis.