A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

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• 作者：Bahgat Fayed (1)
  Ellen Younger (2)
  Gabrielle Taylor (1)
  Margaret C M Smith (1)
  
  1. Department of Biology ; University of York ; York ; YO10 5DD ; UK
  2. School of Medical Sciences ; Institute of Medical Sciences ; University of Aberdeen ; Aberdeen ; AB25 2ZD ; UK
  
• 关键词：Streptomyces ; Cloning ; Integration vector ; Serine integrase ; Bacteriophage ; SV1
• 刊名：BMC Biotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：708 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Life Sciences
  Plant Breeding/Biotechnology
  Stem Cells
  Transgenics
  
• 出版者：BioMed Central
• ISSN：1472-6750

文摘

Background Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. Results We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. Conclusion pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.