Metabolic engineering of Clostridium tyrobutyricum for n-butanol production: effects of CoA transferase

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• 作者：Le Yu ; Jingbo Zhao ; Mengmeng Xu ; Jie Dong…
• 关键词：Aldehyde/alcohol dehydrogenase ; Butanol ; Clostridium tyrobutyricum ; CoA transferase ; Metabolic engineering
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：99
• 期：11
• 页码：4917-4930
• 全文大小：1,994 KB
• 参考文献：Alsaker KV, Spitzer TR, Papoutsakis ET (2004) Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress. J Bacteriol 186:1959-971View Article PubMed Central PubMed
  Alsaker KV, Paredes C, Papoutsakis ET (2010) Metabolite stress and tolerance in the production of biofuels and chemicals: gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum. Biotechnol Bioeng 105:1131-147PubMed
  Bassi D, Fontana C, Gazzola S, Pietta E, Puglisi E, Cappa F, Cocconcelli PS (2013) Draft genome sequence of Clostridium tyrobutyricum strain UC7086, isolated from grana padano cheese with late-blowing defect. Genome Announc 1:e00614-3View Article PubMed Central PubMed
  Bowles LK, Ellefson WL (1985) Effects of butanol on Clostridium acetobutylicum. Appl Environ Microbiol 50:1165-170PubMed Central PubMed
  Branduardi P, De Ferra F, Longo V, Porro D (2014) Microbial n-butanol production from clostridia to non-clostridial hosts. Eng Life Sci 14:16-6View Article
  Chen C-K, Blaschek HP (1999) Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB 8052 solvent production and strain degeneration. Appl Environ Microbiol 65:499-05PubMed Central PubMed
  Cornillot E, Nair RV, Papoutsakis ET, Soucaille P (1997) The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol 179:5442-447PubMed Central PubMed
  Datta R, Zeikus JG (1985) Modulation of acetone butanol ethanol fermentation: utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields. Appl Environ Microbiol 48:764-70
  Demirbas A (2009) Political, economic and environmental impacts of biofuels: a review. Appl Energy 86:s108–s117View Article
  Du Y, Jiang W, Yu M, Tang IC, Yang ST (2015) Metabolic process engineering of Clostridium tyrobutyricum Δack-adhE2 for enhanced n-butanol production from glucose: effects of methyl viologen on NADH availability, flux distribution and fermentation kinetics. Biotechnol Bioeng 112:705-15View Article PubMed
  Dürre P (2007) Biobutanol: an attractive biofuel. Biotechnol J 2:1525-534View Article PubMed
  Dürre P, B?hringer M, Nakotte S, Schaffer S, Thormann K, Zickner B (2002) Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. J Mol Microbiol Biotechnol 4:295-00PubMed
  Fischer RJ, Helms J, Dürre P (1993) Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis. J Bacteriol 175:6959-969PubMed Central PubMed
  Fontaine L, Meynial-Salles I, Girbal L, Yang X, Croux C, Soucaille P (2002) Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol 184:821-30View Article PubMed Central PubMed
  Fox ME, Lemmon MJ, Mauchline ML, Davis TO, Giaccia AJ, Minton NP, Brown JM (1996) Anaerobic bacteria as a delivery system for cancer gene therapy: in vitro activation of 5-fluorocytosine by genetically engineered clostridia. Gene Ther 3:173-78PubMed
  Gerischer U, Dürre P (1990) Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. J Bacteriol 172:6907-918PubMed Central PubMed
  Girbal L, Croux C, Vasconcelos I, Soucaille P (1995) Regulation of metabolic shifts in Clostridium acetobutylicum ATCC 824. FEMS Microbiol Rev 17:287-98View Article
  Green EM (2011) Fermentative production of butanol–the industrial perspective. Curr Opin Biotechnol 22:337-43View Article PubMed
  Han B, Gopalan V, Ezeji C (2011) Acetone production in solventogenic Clostridium species. Appl Microbiol Biotechnol 91:565-76View Article PubMed
  Heap JT, Pennington OJ, Cartman ST, Carter GP, Minton NP (2007) The Clostron: a universal gene knock-out system for the genus Clostridium. J Microbiol Methods 70:452-64View Article PubMed
  Jang YS, Lee JY, Lee J, Park JH, Im JA, Eom MH, Lee J, Lee SH, Song H, Cho JH, Seung DY, Lee SY (2012) Enhanced butanol production obtained by reinforcing the direct butanol-forming route in Clostridium acetobutylicum. MBio 3:e00314-2View Article PubMed Central PubMed
  Janssen H, Grimmler C, Ehrenreich A, Bahl H, Fischer RJ (2012) A transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum—solvent stress caused by a transient n-butanol pulse. J Biotechnol 161:354-65View Article PubMed
  Jiang Y, Xu C, Dong F, Yang Y, Jiang W, Yang S (2009) Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. Metab Eng 11:284-91View Article PubMed
• 作者单位：Le Yu (1)
  Jingbo Zhao (1)
  Mengmeng Xu (1)
  Jie Dong (1)
  Saju Varghese (1)
  Mingrui Yu (1) (2)
  I-Ching Tang (2)
  Shang-Tian Yang (1)
  
  1. William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave., Columbus, OH, 43210, USA
  2. Bioprocessing Innovative Company, 4734 Bridle Path Ct., Dublin, OH, 43017, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

The overexpression of CoA transferase (ctfAB), which catalyzes the reaction: acetate/butyrate + acetoacetyl-CoA ?acetyl/butyryl-CoA + acetoacetate, was studied for its effects on acid reassimilation and butanol biosynthesis in Clostridium tyrobutyricum (Δack, adhE2). The plasmid pMTL007 was used to co-express adhE2 and ctfAB from Clostridium acetobutylicum ATCC 824. In addition, the sol operon containing ctfAB, adc (acetoacetate decarboxylase), and ald (aldehyde dehydrogenase) was also cloned from Clostridium beijerinckii NCIMB 8052 and expressed in C. tyrobutyricum (Δack, adhE2). Mutants expressing these genes were evaluated for their ability to produce butanol from glucose in batch fermentations at pH?5.0 and 6.0. Compared to C. tyrobutyricum (Δack, adhE2) without expressing ctfAB, all mutants with ctfAB overexpression produced more butanol, with butanol yield increased to 0.22??-.26?g/g (vs. 0.10??-.13?g/g) and productivity to 0.35?g/l?h (vs. 0.13?g/l?h) because of the reduced acetate and butyrate production. The expression of ctfAB also resulted in acetone production from acetoacetate through a non-enzymatic decarboxylation.