Bacterial Diversity and Community Structure in Two Bornean Nepenthes Species with Differences in Nitrogen Acquisition Strategies
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- 作者：Wiebke Sickel ; T. Ulmar Grafe ; Ivonne Meuche ; Ingolf Steffan-Dewenter…
- 关键词：Nepenthes ; Carnivorous plants ; Next ; generation sequencing ; 16s rDNA ; Plant ; microbe interactions
- 刊名：Microbial Ecology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：71
- 期：4
- 页码：938-953
- 全文大小：697 KB
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T. Ulmar Grafe (2)
Ivonne Meuche (2)
Ingolf Steffan-Dewenter (1)
Alexander Keller (1)
1. Department of Animal Ecology and Tropical Biology, Biocenter, University of Würzburg, 97074, Würzburg, Germany
2. Faculty of Science, University Brunei Darussalam, Tungku Link, Gadong, BE, 1410, Brunei - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Ecology
Geoecology and Natural Processes
Nature Conservation - 出版者：Springer New York
- ISSN：1432-184X
文摘
Carnivorous plants of the genus Nepenthes have been studied for over a century, but surprisingly little is known about associations with microorganisms. The two species Nepenthes rafflesiana and Nepenthes hemsleyana differ in their pitcher-mediated nutrient sources, sequestering nitrogen from arthropod prey and arthropods as well as bat faeces, respectively. We expected bacterial communities living in the pitchers to resemble this diet difference. Samples were taken from different parts of the pitchers (leaf, peristome, inside, outside, digestive fluid) of both species. Bacterial communities were determined using culture-independent high-throughput amplicon sequencing. Bacterial richness and community structure were similar in leaves, peristomes, inside and outside walls of both plant species. Regarding digestive fluids, bacterial richness was higher in N. hemsleyana than in N. rafflesiana. Additionally, digestive fluid communities were highly variable in structure, with strain-specific differences in community composition between replicates. Acidophilic taxa were mostly of low abundance, except the genus Acidocella, which strikingly reached extremely high levels in two N. rafflesiana fluids. In N. hemsleyana fluid, some taxa classified as vertebrate gut symbionts as well as saprophytes were enriched compared to N. rafflesiana, with saprophytes constituting potential competitors for nutrients. The high variation in community structure might be caused by a number of biotic and abiotic factors. Nitrogen-fixing bacteria were present in both study species, which might provide essential nutrients to the plant at times of low prey capture and/or rare encounters with bats.