Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan
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- 作者：Zareen Fatima (1)
Muhammad Idrees (1)
Mohammad A Bajwa (2)
Zarfishan Tahir (3)
Obaid Ullah (3)
Muhammad Q Zia (2)
Abrar Hussain (1)
Madiha Akram (1)
Bushra Khubaib (1)
Samia Afzal (1)
Saira Munir (1)
Sana Saleem (1)
Bisma Rauff (1)
Sadaf Badar (1)
Mahrukh Naudhani (1)
Sadia Butt (1)
Mahwish Aftab (1)
Liaqat Ali (1)
Muhammad Ali (1) - 刊名：BMC Microbiology
- 出版年：2011
- 出版时间：December 2011
- 年：2011
- 卷：11
- 期：1
- 全文大小：255KB
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Muhammad Idrees (1)
Mohammad A Bajwa (2)
Zarfishan Tahir (3)
Obaid Ullah (3)
Muhammad Q Zia (2)
Abrar Hussain (1)
Madiha Akram (1)
Bushra Khubaib (1)
Samia Afzal (1)
Saira Munir (1)
Sana Saleem (1)
Bisma Rauff (1)
Sadaf Badar (1)
Mahrukh Naudhani (1)
Sadia Butt (1)
Mahwish Aftab (1)
Liaqat Ali (1)
Muhammad Ali (1)
1. Division of Molecular Virology, CEMB University of the Punjab, 87-West Canal Bank Road, Thokar Niaz Baig, Lahore, 53700, Pakistan
2. Department of Gastroenterology, Sheikh Zayed Medical Complex, Lahore, Pakistan
3. Bacteriologist Laboratory, Institute of Public Health, 6-Birdwood Road, Lahore, Pakistan - ISSN：1471-2180
文摘
Background Since the first reported outbreak of dengue hemorrhagic fever in Pakistan, several mini outbreaks have erupted in the region. Dengue virus serotype 3 (DEN-3) was first documented in 2005 outbreak in Karachi. Reports show that serotype 3 is prevalent in Lahore since 2008. Serotype 2 (DEN-2) is the major circulating serotype in Pakistan as it is documented since 1994. We have conducted a detailed study of three outbreaks of dengue virus infection that occurred in years 2007, 2008 and 2009 in Lahore by using molecular techniques such as PCR and nucleotide sequencing of the C-prM gene junction of Dengue virus. Results Through the analysis of 114 serum samples collected over the period of three years (2007-2009), total 20 patients were found to be infected with dengue virus. In year 2007, four were positive for serotype 2 and one sample was positive for serotype DEN-3. In 2008, five samples had concurrent infection with serotypes DEN-2 and DEN-3 while three samples were infected only with serotype DEN-2. In year 2009, one sample had concurrent infection with serotypes DEN-2 and DEN-3 while six were positive for serotype DEN-2 only. Conclusions Our study showed that serotype DEN-2 was dominant in positive samples of dengue virus infection collected during the period of three years (2007-2009). The other serotype present was serotype DEN-3. Genotypes of serotype DEN-2 and serotype DEN-3 were subtype IV and subtype III, respectively.