Expression Analysis of Two P450 Monooxygenase Genes of the Tobacco Cutworm Moth (Spodoptera litura) at Different Developmental Stages and in Response to Plant Allelochemicals

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• 作者：Rui-Long Wang (1) (4)
  Jun Li (1) (4)
  Christian Staehelin (3)
  Xiao-Wei Xin (1) (4)
  Yi-Juan Su (1) (4)
  Ren-Sen Zeng (1) (2) (4)
  
  1. Key Laboratory of Tropical Agro-environment ; Ministry of Agriculture ; South China Agricultural University ; Guangzhou ; 510642 ; China
  4. Key Laboratory of Agroecology and Rural Environment of Guangdong Regular Higher Education Institutions ; South China Agricultural University ; Guangzhou ; 510642 ; China
  3. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources ; School of Life Sciences ; Sun Yat-sen University ; East Campus ; Guangzhou ; 510006 ; China
  2. College of Life Sciences ; Fujian Agriculture and Forestry University ; Fuzhou ; 350002 ; China
  
• 关键词：Spodoptera litura ; Plant allelochemicals ; Cytochrome P450 monooxygenases ; CYP6B48 ; CYP6B58 ; Lepidoptera ; Noctuidae generalist insect pest
• 刊名：Journal of Chemical Ecology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：41
• 期：1
• 页码：111-119
• 全文大小：784 KB
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• 刊物主题：Ecology; Biochemistry, general; Entomology; Biological Microscopy; Agriculture;
• 出版者：Springer US
• ISSN：1573-1561

文摘

Cytochrome P450 monooxygenases (P450s) of insects are known to be involved in the metabolism or detoxification of plant allelochemicals and insecticides. Spodoptera litura (Lepidoptera, Noctuidae) is a polyphagous moth responsible for severe yield losses in many crops. In this study, two full-length P450 genes, CYP6B48 and CYP6B58, were cloned from S. litura. The cDNA sequences encode proteins with 503 and 504 amino acids, respectively. Phylogenetic analysis revealed that CYP6B48 and CYP6B58 belong to the CYP6B subfamily of P450s. Quantitative real-time PCR analyses showed that CYP6B48 and CYP6B58 were expressed only at larval stage, but not at pupal and adult stages. The highest levels of transcripts were found in the midguts and fat bodies of the larvae. No expression was detected in the ovary or hemolymph. Feeding with diets containing cinnamic acid, quercetin, or coumarin did not affect expression of CYP6B48. In contrast, diet supplemented with xanthotoxin dramatically increased the levels of CYP6B48 transcript in the midgut and fat bodies. Larvae fed with flavone had high levels of transcript of CYP6B48 in the midgut, whereas only slightly elevated levels were found in the fat bodies. Effects of the tested allelochemicals on CYP6B58 expression were minor. Hence, our findings show that S. litura responds to specific allelochemicals such as xanthotoxin with the accumulation of CYP6B48 transcripts, suggesting that specific signals in the food control the insect鈥檚 ability to convert toxic allelochemicals to less harmful forms at the transcriptional level.