Sensitization of U937 leukemia cells to doxorubicin by the MG132 proteasome inhibitor induces an increase in apoptosis by suppressing NF-kappa B and mitochondrial membrane potential loss
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- 作者：Pablo César Ortiz-Lazareno (1)
Alejandro Bravo-Cuellar (1) (2)
José Manuel Lerma-Díaz (1) (2)
Luis Felipe Jave-Suárez (1)
Adriana Aguilar-Lemarroy (1)
Jorge Ramiro Domínguez-Rodríguez (1) (3)
Oscar González-Ramella (4)
Ruth De Célis (1)
Paulina Gómez-Lomelí (1) (5)
Georgina Hernández-Flores (1) - 关键词：Apoptosis ; NF ; кB ; Caspase activation ; Mitochondrial dysfunction ; Senescence ; MG132 ; Doxorubicin ; Leukemia
- 刊名：Cancer Cell International
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：610 KB
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Alejandro Bravo-Cuellar (1) (2)
José Manuel Lerma-Díaz (1) (2)
Luis Felipe Jave-Suárez (1)
Adriana Aguilar-Lemarroy (1)
Jorge Ramiro Domínguez-Rodríguez (1) (3)
Oscar González-Ramella (4)
Ruth De Célis (1)
Paulina Gómez-Lomelí (1) (5)
Georgina Hernández-Flores (1)
1. División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, México
2. Centro Universitario de los Altos, Universidad de Guadalajara (UdeG), Tepatitlán de Morelos, Jalisco, México
3. Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingeniería, UdeG, Kragujevac, Jalisco, México
4. Servicio de Hemato-Oncología Pediatría, OPD Hospital Civil Juan I. Menchaca, Guadalajara, Jalisco, México
5. Programa de Doctorado en Ciencias Biomédicas Orientación Inmunología, Centro Universitario de Ciencias de la Salud, UdeG, Kragujevac, Jalisco, México - ISSN：1475-2867
文摘
Background The resistance of cancerous cells to chemotherapy remains the main limitation for cancer treatment at present. Doxorubicin (DOX) is a potent antitumor drug that activates the ubiquitin-proteasome system, but unfortunately it also activates the Nuclear factor kappa B (NF-кB) pathway leading to the promotion of tumor cell survival. MG132 is a drug that inhibits I kappa B degradation by the proteasome-avoiding activation of NF-кB. In this work, we studied the sensitizing effect of the MG132 proteasome inhibitor on the antitumor activity of DOX. Methods U937 human leukemia cells were treated with MG132, DOX, or both drugs. We evaluated proliferation, viability, apoptosis, caspase-3, -8, and ? activity and cleavage, cytochrome c release, mitochondrial membrane potential, the Bcl-2 and Bcl-XL antiapoptotic proteins, senescence, p65 phosphorylation, and pro- and antiapoptotic genes. Results The greatest apoptosis percentage in U937 cells was obtained with a combination of MG132-?DOX. Likewise, employing both drugs, we observed a decrease in tumor cell proliferation and important caspase-3 activation, as well as mitochondrial membrane potential loss. Therefore, MG132 decreases senescence, p65 phosphorylation, and the DOX-induced Bcl-2 antiapoptotic protein. The MG132-?DOX treatment induced upregulation of proapoptotic genes BAX, DIABLO, NOXA, DR4, and FAS. It also induced downregulation of the antiapoptotic genes BCL-XL and SURVIVIN. Conclusion MG132 sensitizes U937 leukemia cells to DOX-induced apoptosis, increasing its anti-leukemic effectiveness.