Thidiazuron induces high-frequency indirect shoot organogenesis of Bienertia sinuspersici: a single-cell C4 species
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- 作者：Jennifer Anne Northmore ; Dustin Sigurdson…
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：126
- 期：1
- 页码：141-151
- 全文大小：2,194 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1573-5044
- 卷排序：126
文摘
Induction of high-frequency shoot organogenesis using stem-derived callus of Bienertia sinuspersici was achieved on Murashige and Skoog basal medium containing 0.0045–9.0 μM thidiazuron (TDZ). Other cytokinins [kinetins, and 6-benzyladenine (BA)] were also tested for their shoot induction potential but only TDZ was effective at promoting shoot morphogenesis and development. The highest shoot induction efficiency (80 %) was obtained on a medium containing 0.45 μM TDZ. The TDZ-derived shoots were transferred to medium containing 8.8 μM BA for multiplication followed by gibberellic acid (1.45–14.5 μM) treatment to promote shoot elongation. The elongated shoots spontaneously developed adventitious roots during the elongation process. The rooted plantlets were successfully transplanted to soil, acclimated in the greenhouse and then transferred to a growth chamber under controlled conditions. Histological analysis of leaf anatomy of the regenerated plants showed that the distinctive cytoplasmic compartmentation feature of the single-cell C4 photosynthetic system was re-established. Moreover, Western blots detected high levels of key C4 enzymes in mature leaves of TDZ-derived shoots in vitro and the regenerated plants. Overall, these findings show that in vitro regenerated plants possessed the single-cell C4 leaf anatomy and C4 enzymes comparable to those of the seed-derived plants.KeywordsBienertia sinuspersiciIndirect organogenesisSingle-cell C4 photosynthesisRegenerationThidiazuron