NMR Techniques in Metabolomic Studies: A Quick Overview on Examples of Utilization

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• 作者：Joanna Kruk ; Marek Doskocz ; Elżbieta Jodłowska…
• 刊名：Applied Magnetic Resonance
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：48
• 期：1
• 页码：1-21
• 全文大小：1338KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Solid State Physics; Spectroscopy/Spectrometry; Atoms and Molecules in Strong Fields, Laser Matter Interaction; Physical Chemistry; Organic Chemistry;
• 出版者：Springer Vienna
• ISSN：1613-7507
• 卷排序：48

文摘

Metabolomics is a rapidly developing branch of science that concentrates on identifying biologically active molecules with potential biomarker properties. To define the best biomarkers for diseases, metabolomics uses both models (in vitro, animals) and human, as well as, various techniques such as mass spectroscopy, gas chromatography, liquid chromatography, infrared and UV–VIS spectroscopy and nuclear magnetic resonance. The last one takes advantage of the magnetic properties of certain nuclei, such as 1H, 13C, 31P, 19F, especially their ability to absorb and emit energy, what is crucial for analyzing samples. Among many spectroscopic NMR techniques not only one-dimensional (1D) techniques are known, but for many years two-dimensional (2D, for example, COSY, DOSY, JRES, HETCORE, HMQS), three-dimensional (3D, DART-MS, HRMAS, HSQC, HMBC) and solid-state NMR have been used. In this paper, authors taking apart fundamental division of nuclear magnetic resonance techniques intend to shown their wide application in metabolomic studies, especially in identifying biomarkers.