Antimicrobial activity of Alcaligenes sp. HPC 1271 against multidrug resistant bacteria

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• 作者：Atya Kapley ; Himgouri Tanksale ; Sneha Sagarkar…
• 关键词：Alcaligenes ; Antimicrobial ; Draft genome ; Multidrug resistant ; Tunicamycin
• 刊名：Functional & Integrative Genomics
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：16
• 期：1
• 页码：57-65
• 全文大小：1,895 KB
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• 作者单位：Atya Kapley (1) (3)
  Himgouri Tanksale (1)
  Sneha Sagarkar (1)
  A. R. Prasad (2)
  Rathod Aravind Kumar (2)
  Nandita Sharma (1)
  Asifa Qureshi (1)
  Hemant J. Purohit (1)
  
  1. CSIR-NEERI, Nehru Marg, Nagpur, 440020, Maharashtra, India
  3. Environmental Genomics Division, National Environmental Engineering Research Institute, CSIR, Nehru Marg, Nagpur, 440 020, India
  2. CSIR-IICT, Uppal Road, Tarnaka, Hyderabad, 500007, Andhra Pradesh, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Genetics and Genomics
  Microbial Genetics and Genomics
  Biochemistry
  Bioinformatics
  Animal Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-7948

文摘

Alcaligenes sp. HPC 1271 demonstrated antibacterial activity against multidrug resistant bacteria, Enterobacter sp., resistant to sulfamethoxazole, ampicillin, azithromycin, and tetracycline, as well as against Serratia sp. GMX1, resistant to the same antibiotics with the addition of netilmicin. The cell-free culture supernatant was analyzed for possible antibacterials by HPLC, and the active fraction was further identified by LC-MS. Results suggest the production of tunicamycin, a nucleoside antibiotic. The draft genome of this bacterial isolate was analyzed, and the 4.2 Mb sequence data revealed six secondary metabolite-producing clusters, identified using antiSMASH platform as ectoine, butyrolactone, phosphonate, terpene, polyketides, and nonribosomal peptide synthase (NRPS). Additionally, the draft genome demonstrated homology to the tunicamycin-producing gene cluster and also defined 30 ORFs linked to protein secretion that could also play a role in the antibacterial activity observed. Gene expression analysis demonstrated that both NRPS and dTDP-glucose 4,6-dehydratase gene clusters are functional and could be involved in antibacterial biosynthesis. Keywords Alcaligenes Antimicrobial Draft genome Multidrug resistant Tunicamycin