Production and characterization of Monoclonal antibodies to bluetongue virus
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- 作者：Veerakyathappa Bhanuprakash (1)
Madhusudhan Hosamani (3)
Vinayagamurthy Balamurugan (2)
Pradeep Narayan Gandhale (1)
Gnanavel Venkatesan (1)
Raj Kumar Singh (4) - 关键词：Bluetongue virus ; Competitive ELISA ; Enzyme ; linked immunosorbent assay ; Monoclonal antibody ; India
- 刊名：Virologica Sinica
- 出版年：2011
- 出版时间：February 2011
- 年：2011
- 卷：26
- 期：1
- 页码：8-18
- 全文大小：636KB
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17. Wechsler S J, McHolland L E. 1988. Susceptibilities of 14 cell lines to bluetongue virus infection. J Clin Microbiol, 26: 2324鈥?327. - 作者单位：Veerakyathappa Bhanuprakash (1)
Madhusudhan Hosamani (3)
Vinayagamurthy Balamurugan (2)
Pradeep Narayan Gandhale (1)
Gnanavel Venkatesan (1)
Raj Kumar Singh (4)
1. Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263 138, Nainital (Distt.) Uttarakhand, India
3. Indian Veterinary Research Institute, H A Farm, Hebbal, Bangalore, 560 024, Karnataka, India
2. Project Directorate on Animal Disease Monitoring And Surveillance, H A Farm, Hebbal, Bangalore, 560 024, Karnataka, India
4. National Research Centre on Equines and Veterinary Type Culture Centre, Sirsa Road, Hisar, 125 001, Haryana, India - ISSN：1995-820X
文摘
In the present study, a total of 24 MAbs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres, isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones, a majority of them (n = 18) belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA, the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However, this clone showed a variable percent of inhibition ranging from16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones, only 4A10 was found to have a possible diagnostic application.