The influence of the p53 gene on the in vitro chemosensitivity of colorectal cancer cells
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- 作者：Minhua Zheng ; Hao Wang ; Haobo Zhang ; Qishi Ou ; Baihua Shen ; Ningli Li and Baoming Yu
- 关键词：Key words Colorectal neoplasms ; Apoptosis ; Chemosensitivity ; p53 gene
- 刊名：Journal of Cancer Research and Clinical Oncology
- 出版年：1999
- 出版时间：May 1999
- 年：1999
- 卷：125
- 期：6
- 页码：357-360
- 全文大小：77 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Oncology
Cancer Research
Internal Medicine
Hematology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-1335
文摘
Purpose : The p53 gene is considered one of the most important in the control of apoptosis, and its mutations have a close relationship with chemosensitivity. The aim of this work was to investigate the role of p53 in the apoptosis of colorectal cancer cells in vitro, induced by 5-fluorouracil (5-FU) and hydroxy-camptothecin (HCPT). Methods : A total of 39 colorectal cancer samples from patients were treated in vitro with 5-FU (10 μg/ml), 5-FU (10 μg/ml) + leucovorin (5 μg/ml), HCPT (0.1 μg/ml) and HCPT (0.1 μg/ml) + Salvia mitorrhiza (6 μl), using an in situ terminal deoxynucleotidyltransferase assay to detect chemosensitivity. p53 gene mutations from tumor DNA were detected, after amplification by the polymerase chain reaction of exons 5–8, by non-radioactive single-strand conformation polymorphism. Results : p53 gene mutations were observed in 43.6% (17/39) of colorectal carcinomas, when the terminal deoxynucleotidyltransferase assay was used to detect the tumor apoptotic rate. Cells with mutated p53 had lower chemosensitivity than those without (p < 0.01). Conclusion : Routine assessment of p53 status may be helpful in selecting patients with the wild-type p53 gene, who have a predictably better response to chemotherapy.