Assessment of Isoprostanes in Human Plasma: Technical Considerations and the Use of Mass Spectrometry

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• 作者：Yiu Yiu Lee ; Jean-Marie Galano ; Camille Oger ; Claire Vigor ; Reversat Guillaume…
• 关键词：Lipid peroxidation ; Isoprostanes ; Neuroprostanes ; GC–MS ; LC–MS/MS
• 刊名：Lipids
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：51
• 期：11
• 页码：1217-1229
• 全文大小：664 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Life Sciences
  Biochemistry
  Medicinal Chemistry
  Microbial Genetics and Genomics
  Nutrition
  Bioorganic Chemistry
  Medical Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9307
• 卷排序：51

文摘

Oxygenated lipid mediators released from non-enzymatic peroxidation of polyunsaturated fatty acids (PUFA) are known to have functional roles in humans. Notably, among these lipid mediators, isoprostanes molecules are robust biomarkers of oxidative stress but those from n-3 PUFA are also bioactive molecules. In order to identify and assess the isoprostanes, the use of mass spectrometry (MS) for analysis is preferable and has been used for over two decades. Gas chromatography (GC) is commonly coupled to the MS to separate the derivatized isoprostanes of interest in biological samples. In order to increase the accuracy of the analytical performance, GC–MS/MS was also applied. Lately, MS or MS/MS has been coupled with high-performance liquid chromatography to assess multiple isoprostane molecules in a single biological sample without derivatization process. However, there are limitations for the use of LC–MS/MS in the measurement of plasma isoprostanes, which will be discussed in this review.