Improved Alkane Production in Nitrogen-Fixing and Halotolerant Cyanobacteria via Abiotic Stresses and Genetic Manipulation of Alkane Synthetic Genes

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• 作者：Hakuto Kageyama ; Rungaroon Waditee-Sirisattha ; Sophon Sirisattha…
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：71
• 期：1
• 页码：115-120
• 全文大小：800 KB
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  10.Waditee-Sirisattha R, Singh M, Kageyama H, Sittipol D, Rai AK, Takabe T (2012) Anabaena sp. PCC7120 transformed with glycine methylation genes from Aphanothece halophytica synthesized glycine betaine showing increased tolerace to salt. Arch Microbiol 194:909鈥?14PubMed View Article
  11.Waditee-Sirisattha R, Kageyama H, Sopun W, Tanaka Y, Takabe T (2014) Identification and up-regulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress in halotolerant cyanobacterium Aphanothece halophytica. Appl Environ Microbiol 80:1763鈥?769PubMed Central PubMed View Article
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• 作者单位：Hakuto Kageyama (1)
  Rungaroon Waditee-Sirisattha (2)
  Sophon Sirisattha (3)
  Yoshito Tanaka (1)
  Aparat Mahakhant (3)
  Teruhiro Takabe (1) (4)
  
  1. Graduate School of Environmental and Human Sciences, Meijo University, Nagoya, 468-8502, Japan
  2. Department of Microbiology, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand
  3. Thailand Institute of Scientific and Technological Research, Pathum Thani, 12120, Thailand
  4. Research Institute of Meijo University, Nagoya, 468-8502, Japan
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Cyanobacteria possess the unique capacity to produce alkane. In this study, effects of nitrogen deficiency and salt stress on biosynthesis of alkanes were investigated in three kinds of cyanobacteria. Intracellular alkane accumulation was increased in nitrogen-fixing cyanobacterium Anabaena sp. PCC7120, but decreased in non-diazotrophic cyanobacterium Synechococcus elongatus PCC7942 and constant in a halotolerant cyanobacterium Aphanothece halophytica under nitrogen-deficient condition. We also found that salt stress increased alkane accumulation in Anabaena sp. PCC7120 and A. halophytica. The expression levels of two alkane synthetic genes were not upregulated significantly under nitrogen deficiency or salt stress in Anabaena sp. PCC7120. The transformant Anabaena sp. PCC7120 cells with additional alkane synthetic gene set from A. halophytica increased intracellular alkane accumulation level compared to control cells. These results provide a prospect to improve bioproduction of alkanes in nitrogen-fixing halotolerant cyanobacteria via abiotic stresses and genetic engineering.