Study of the gastrointestinal parasitic fauna of captive non-human primates (Macaca fascicularis)

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• 作者：Sergio Aurelio Zanzani ; Alessia Libera Gazzonis ; Sara Epis…
• 关键词：Trichuris sp. ; Protozoans ; Parasites ; Oesophagostomum sp. ; Macaca fascicularis ; Blastocystis sp.
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：115
• 期：1
• 页码：307-312
• 全文大小：284 KB
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• 作者单位：Sergio Aurelio Zanzani (1)
  Alessia Libera Gazzonis (1)
  Sara Epis (1)
  Maria Teresa Manfredi (1)
  
  1. Department of Veterinary Science and Public Health, Università degli Studi di Milano, via Celoria 10, 20133, Milan, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

The aim of this study was to examine helminths and protozoans in cynomolgus macaques (Macaca fascicularis) imported from registered breeding facilities in China and their relation to health risks for non-human primate handlers in biomedical research centers and in breeding facilities. Fresh fecal samples were collected from a total of 443 M. fascicularis and analyzed by copromicroscopical analysis, immunoenzymatic, or molecular assays. As to helminths, whose eggs were shed in 2.03% of the samples, Trichuris and Oesophagostomum were the only two taxa found, with low prevalence and low eggs per gram (EPG) values. Protozoans were more frequently detected (87.40%), with Entamoeba coli (85.19%) and Endolimax nana (79.26%) as the most prevalent species shed. Other parasites found by fecal smear examination were uninucleated-cyst-producing Entamoebas (78.52%), Iodamoeba bütschlii (42.96%), and Chilomastix mesnili (24.44%), while cysts of Balantidium coli (22.2%) were only observed by sedimentation. No coproantigens of Giardia duodenalis, Cryptosporidium spp., and Entamoeba histolytica complex were detected. Blastocystis sp. infection was noticed in 87.63% of macaques by PCR. These cynomolgus monkeys were infected with many subtypes (ST1, ST2, ST3, ST5, and ST7), where the predominant Blastocystis sp. subtypes were ST2 (77.5%), followed by ST1 (63.5%). Data collected confirmed the presence of potentially zoonotic parasites and a high parasite diversity, suggesting the need for appropriate and sensitive techniques to adequately control them and related health risks for handlers of non-human primates in biomedical research centers and in breeding facilities.