Determination of Trace Vitamin D in Milk Samples by Graphene-Based Magnetic Solid-Phase Extraction Method Coupled with HPLC

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• 作者：Zhe Jiao ; Shaohe Jiao ; Zongning Guo ; Hongwei Chen ; Ni Zhang…
• 关键词：Magnetic solid ; phase extraction ; Fe3O4@graphene ; Vitamin D ; HPLC
• 刊名：Food Analytical Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：10
• 期：3
• 页码：820-826
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Food Science; Chemistry/Food Science, general; Microbiology; Analytical Chemistry;
• 出版者：Springer US
• ISSN：1936-976X
• 卷排序：10

文摘

In this paper, a kind of simple and environmental friendly sample pretreatment method was developed for analysis of vitamin D in milk samples. Without saponification or protein precipitation, vitamin D₂ and vitamin D₃ could be captured directly from milk samples by graphene-coated magnetic particle (Fe₃O₄@graphene) and separated by magnetic field with only 0.5-mL desorption solvent. The vitamin D₂ and vitamin D₃ were then detected by high-performance liquid chromatography (HPLC). The main effect parameters including extraction time, solution ionic strength, desorption solvent, and desorption time were investigated. Under optimized conditions, this method showed low detection limits (0.03 ng/mL for vitamin D₂ and 0.08 ng/mL for vitamin D₃), wide linearity, and good precision (2.7–3.6 % for intraday, 2.3–4.5 % for inter-day). The matrix interference-produced milk could be effectively eliminated using this method, and satisfactory recoveries of the spiked samples were 75.5 to 89.3 %. The results indicated that the Fe₃O₄@graphene can be favorably used for the extraction of the vitamin D in milk samples. The reused material displayed a long-term stability after undergoing extraction of 100 times.