In-vivo and label-free imaging of cellular and tissue structures in mouse ear skin by using second- and third-harmonic generation microscopy

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• 作者：Eung Jang Lee (1)
  Boram Kim (1)
  Hong-Gyu Ahn (1)
  Seung-Han Park (1)
  Eunji Cheong (2)
  Sangyoup Lee (3)
  
  1. Department of Physics ; Yonsei University ; Seoul ; 120-749 ; Korea
  2. Department of Biotechnology ; Yonsei University ; Seoul ; 120-749 ; Korea
  3. Biomedical Research Institute ; Korea Institute of Science and Technology ; Seoul ; 136-791 ; Korea
  
• 关键词：SHG and THG microscopy ; In ; vivo imaging ; Label ; free imaging ; Cellular and tissue structures ; Video ; rate microscope
• 刊名：Journal of the Korean Physical Society
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：66
• 期：4
• 页码：597-601
• 全文大小：1,899 KB
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• 刊物主题：Physics, general; Theoretical, Mathematical and Computational Physics; Particle and Nuclear Physics;
• 出版者：Springer Netherlands
• ISSN：1976-8524

文摘

A video-rate multimodal microscope, which can obtain second- and third- harmonic generation (SHG and THG) images simultaneously, is developed for investigating cellular and tissue structures in mouse ear skin. By utilizing in-vivo video-rate epi-detected SHG and THG microscopy, we successfully demonstrate that combined images of subcutaneous cellular components and peripheral nerve fibers, together with the collagen fiber, in the mouse ear pinna can be obtained without employing fluorescent probes. We also show that the flow of red blood cells and the diameter change of arteriole-like blood vessels can be visualized with femtosecond laser pulses with a wavelength of 1036 nm. In particular, the epi-THG contrast images of the blood-vessel walls display clearly the difference between the arteriole-like and the venule capillary-like blood-vessel types. We should emphasize that our newly-developed microscope system has a unique feature in that it can produce simultaneous in-vivo label-free SHG and THG images in contrast to the conventional confocal and two-photon microscopes.