Cause of the abnormal acidity of ionogenic groups of the lysozyme active center in cell wall lysis Micrococcus lysodeicticus

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• 作者：O. I. Smotrov (1)
  V. M. Borzenkov (1) vmborzenkov@mail.ru
  V. I. Surovtsev (1)
• 关键词：lysozyme substrate – alkaline shift – ionogenic group – immobilized substrate
• 刊名：Russian Journal of Bioorganic Chemistry
• 出版年：2011
• 出版时间：September 2011
• 年：2011
• 卷：37
• 期：5
• 页码：564-568
• 全文大小：201.7 KB
• 参考文献：1. Blake, C.C.F., Mair, G.A., North, A.C.T., and Phyllips, D.S., Sarma, U.R, Proc. Roy. Soc. B, 1967, vol. 167, pp. 365–377.
  2. Strier, L., Biokhimiya (Biochemistry), Moscow: Mir, 1984.
  3. Neuberger, A. and Wilson, B., Biochim. Biophys. Acta, 1967, vol. 147, pp. 473–486.
  4. Klesov, A.A., Rabinovich, M.L., and Berezin, I.V., Bioorg. Khim., 1976, vol. 2, pp. 795–802.
  5. Berezin, I.V. and Klesov, A.A., Prakticheskii kurs khimicheskoi i fermentativnoi kinetiki (Practical Handbook of Chemical and Enzyme Kinetics), Moscow: Mosk. Gos. Univ., 1976.
  6. Westlee, J., Fermentativnyi kataliz (Enzymatic Catyalysis), Moscow: Mir, 1972.
  7. Dyson, J.E.D. and Noltmann, E.A., J. Biol. Chem., 1971, vol. 93, pp. 5882–5887.
  8. Surovtsev, V.I., Borzenkov, V.M., Fedorov, T.V., and Smotrov, O.I., Biokhimiya, 2007, vol. 72, pp. 1214–1219.
  9. Goldstein, L., Levin, Y., and Katchalski, E., Biochemistry, 1964, vol. 3, pp. 1913–1919.
  10. Strominger, J.L. and Ghyisen, J.-M., Science, 1967, vol. 156, pp. 213–221.
  11. Yakovlev, V.A., Kinetika fermentativnogo kataliza (Kinetics of Enzymatic Catalysis), Moscow: Nauka, 1965.
  12. Cornish-Bowden, E., Osnovy fermentativnoi kinetiki (Principles of Enzymatic Kinetics), Moscow: Mir, 1979.
  13. Surovtsev, V.I., Borzenkov, V.M., Khatyushin, Yu.I., and Teimurazov, M.G., RF Patent 2342431, Byull. Izobret., 2008, no. 36.
  14. Shugar, D., Biochim. Biophys. Acta, 1952, vol. 8, pp. 302–306.
• 作者单位：1. State Research Center for Applied Microbiology and Biotechnology, Ministry of Health and Social Development of the Russian Federation, Obolensk, Moscow oblast, 142279 Russia
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Organic Chemistry
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-330X

文摘

The influence of pH within the range 6.9–10.0 on the kinetic parameters of Micrococcus lysodeicticus cell lysis catalyzed by hen egg lysozyme has been studied at 25°C and 37°C. The effective pK _b values have been calculated for the group determining lysozyme catalytic activity. The ΔH _ion value indicates that this group is a carboxyl, although its pK (9.15 at 25°C) is far beyond the range characteristic of carboxylic groups. The cause of this abnormal pK _b value is supposed to be the strong negative charge of the bacterial cell wall. As a result, the enzyme, which catalyzes the hydrolysis of N-acetylglucosamine-N-acetylmuramic acid copolymer, operates in a highly acidic microenvironment.