Purification of bacteriocins by chromatographic methods

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• 作者：V. I. Surovtsev ; V. M. Borzenkov ; V. P. Levchuk
• 关键词：bacteriocins ; hydrophobicity ; ion ; change and hydrophobic chromatography ; membranes ; purification ; surface cell fragments
• 刊名：Applied Biochemistry and Microbiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：51
• 期：9
• 页码：881-886
• 全文大小：420 KB
• 参考文献：1.Jankauskas, S.S., Yankauskas, S.S., Plotnikov, E.Yu., Morosanova, M.A., Pevzner, I.B., Zorova, L.D., Skulachev, V.P., and Zorov, D.B., Mitochondria-targeted antioxidant SkQR1 ameliorates gentamycininduced renal failure and hearing loss, Biochemistry (Moscow), 2012, vol. 77, no. 6, pp. 666鈥?70.CrossRef
  2.Cleveland, J., Cleveland, J., Montville, T.J., Nes, I.F., and Chikindas, M.L., Int. J. Food Microbiol., 2001, vol. 71, no. 1, pp. 1鈥?0.CrossRef PubMed
  3.Bilkov谩, A. and Bala鈥檢ov谩, A., Bacteriocins produced by lactic acid bacteria, Ceska Slov. Farm., 2011, vol. 60, no. 1, pp. 65鈥?2.PubMed
  4.Nicolas, G.G., Lapointe, G., and Lavoie, M.C., Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1, BMC Microbiol., 2011, vol. 11, no. 1, pp. 69鈥?8.PubMedCentral CrossRef PubMed
  5.Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 1970, vol. 227, pp. 680鈥?85.CrossRef PubMed
  6.Riley, M.A. and Wertz, J.E., Bacteriocins, evolution, ecology and application, Annu. Rev. Microbiol., 2002, vol. 7, pp. 129鈥?33.
  7.Scopes, R., Protein Purification: Principles and Practice, New York: Springer, 1982.CrossRef
  8.Carolissen-Mackay, V., Carolissen-Mackay, V., Arendse, G., and Hastings, J., Purification of bacteriocins of lactic acid bacteria: problems and pointers, Int. J. Food Microbiol., 1997, vol. 34, no. 1, pp. 1鈥?6.CrossRef PubMed
  9.Brock, J., Poluchenie preparatov immunoglobulinov. Immunologicheskie metody (Obtaining Immunoglobulin Preparations. Immunological Methods), Frimel鈥? G., Ed., Moscow: Meditsina, 1980.
  10.Klesov, A.A., Rabinovich, M.L., and Berezin, I.V., Kinetics of enzymatic reactions in heterogeneous systems, Bioorg. Khim., 1976, vol. 2, no. 6, pp. 680鈥?88.
  11.Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Levchuk, V.P., and Seal, B.S., Isolation of Lactobacillus salivarius strain and purification of its bacteriocin which is inhibitory to Campylobacter jejuni in the chicken gastrointestinal system, Antimicrob. Agents Chemother., 2006, vol. 50, pp. 3111鈥?116.PubMedCentral CrossRef PubMed
  12.Lur鈥檈, A.A., Sorbenty i khromatograficheskie nositeli (Sorbents and Chromatographic Media), Moscow: Khimiya, 1972.
  13.Scopes, R.K., Measurement of protein by spectrophotometry at 205 nm, Anal. Biochem., 1974, vol. 59, pp. 277鈥?82.CrossRef PubMed
  
• 作者单位：V. I. Surovtsev (1)
  V. M. Borzenkov (1)
  V. P. Levchuk (1)
  
  1. State Research Center for Applied Microbiology and Biotechnology, Moskovskaya Oblast, Obolensk, 142269, Russia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Microbiology
  Medical Microbiology
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3024

文摘

The bacteriocins secreted by the cells of two B. subtilis strains have been isolated by hydrophobic or ion-exchange chromatography on CMC followed by purification on membranes. The yields of electrophoretically pure products were ~75 % of the total activity in the supernatant of culture fluid obtained after cell sedimentation. The bacteriocin secreted by B. subtilis BSX strain cells has high hydrophobicity, and it is mainly found on the surface of cell walls in the culture fluid. These fragments served as sorbents in hydrophobic chromatography. A B. subtilis strain B-112 bacteriocin predominantly occurs in the free state in the culture fluid supernatant. It was purified after the removal of cell debris on CMC and was then eluted with a glycine-HCl buffer at pH 2.5. The concentration of the purified bacteriocins was determined at 205 nm; therefore, the method did not require aromatic amino acids to be available in the macromolecule structure. It is believed that these chromatographic techniques can be used for purification with a high yield of bacteriocins of other producers. Keywords bacteriocins hydrophobicity ion-change and hydrophobic chromatography membranes purification surface cell fragments