The cytoprotective effect of Rumex Aquaticus Herba extract against hydrogen peroxide-induced oxidative stress in AGS cells

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• 作者：Eun Jeong Cho ; Seung In Um ; Jeong Hoon Han ; Byeonghee Kim…
• 关键词：Herbal extract ; Oxidative stress ; Antioxidant ; Anti ; inflammatory ; Cytoprotection
• 刊名：Archives of Pharmacal Research
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：39
• 期：12
• 页码：1739-1747
• 全文大小：
• 刊物主题：Pharmacy; Pharmacology/Toxicology;
• 出版者：Pharmaceutical Society of Korea
• ISSN：1976-3786
• 卷排序：39

文摘

The Rumex Aquaticus Herba extract containing quercetin-3-β-D-glucuronopyranoside (ECQ) has been reported to exhibit various pharmacological activities, including anti-inflammatory and anti-oxidative effects. This plant has been traditionally used for the treatment of diarrhea, disinfestation, edema and jaundice, and as an antipyretic drug. The aim of the present study was to investigate the ability of ECQ to protect against oxidative damage and to determine its signaling mechanism in AGS cells. The protein expressions of heme oxygenase-1 (HO-1) and nuclear factor-erythroid 2 related factor 2 (Nrf2) were measured by Western blots. Cell viability was measured by MTT assay. Intracellular reactive oxygen species (ROS) levels were measured using 2′,7′-dichlorofluorescein diacetate. Glutathione peroxidase levels were measured using kits. The protein expressions of HO-1 and its upstream mediator, Nrf2, increased after ECQ treatment. The HO-1 inhibitor, ZnPP, repressed the protective effect of ECQ on H₂O₂-induced cell damage. We found that LY294002, a specific PI3 K/Akt inhibitor, suppressed ECQ-induced HO-1 expression. ECQ significantly attenuated H₂O₂-induced cytotoxicity and ROS generation. Also, ECQ enhanced the antioxidant enzyme activities of glutathione peroxidase. These results suggest that ECQ exerts a cytoprotective effect against H₂O₂-induced oxidative stress by upregulation of Nrf2/HO-1 via the PI3 K/Akt pathway.