In Vitro Effect of Amphotericin B on Candida albicans, Candida glabrata and Candida parapsilosis Biofilm Formation

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• 作者：Ma?gorzata Pra?yńska (1)
  Eugenia Gospodarek (1)
  
• 关键词：Antifungals ; Candidosis ; Polyenes ; Sessile cells
• 刊名：Mycopathologia
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：177
• 期：1-2
• 页码：19-27
• 全文大小：229 KB
• 作者单位：Ma?gorzata Pra?yńska (1)
  Eugenia Gospodarek (1)
  
  1. Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 9 Maria Sk?odowska-Curie Street, 85-094, Bydgoszcz, Poland
  
• ISSN：1573-0832

文摘

Candida spp. biofilm is considered highly resistant to conventional antifungals. The aim of this study was to investigate the in vitro effect of amphotericin B on Candida spp. biofilms at different stages of maturation. We investigated the activity of amphotericin B against 78 clinical isolates of Candida spp., representing three species, growing as planktonic and sessile cells, by a widely accepted broth microdilution method. The in vitro effect on sessile cell viability was evaluated by MTT reduction assay. All examined strains were susceptible to amphotericin B when grown as free-living cells. At the early stages of biofilm maturation 96.7-00.0?% strains, depending on species, displayed amphotericin B sessile minimal inhibitory concentration (SMIC) ??μg/mL. Mature Candida spp. biofilm of 32.1-0.0?% strains displayed amphotericin B SMIC ??μg/mL. Based on these results, amphotericin B displays species- and strain-depending activity against Candida spp. biofilms.