Diversity of fungi isolated from three temperate ascidians

详细信息    查看全文

• 作者：Susanna López-Legentil ; Patrick M. Erwin ; Marta Turon ; Oded Yarden
• 关键词：Fungus ; Mediterranean Sea ; ITS ; Sea ; squirt ; Tunicata ; Pathogens
• 刊名：Symbiosis
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：66
• 期：2
• 页码：99-106
• 全文大小：667 KB
• 参考文献：Ainsworth TD, Thurber RV, Gates RD (2010) The future of coral reefs: a microbial perspective. Trends Ecol Evol 25:233-40CrossRef PubMed
  Ainsworth TD, Krause L, Bridge T, Torda G, Raina J-B, Zakrzewski M, Gates RD, Padilla-Gamino JL, Spalding HL, Smith C, Woolsey ES, Bourne DG, Bongaerts P, Hoegh-Guldberg O, Leggat W (2015) The coral core microbiome identifies rare bacterial taxa as ubiquitous endosymbionts. ISME J in press
  Alker AP, Smith GW, Kim K (2001) Characterization of Aspergillus sydowii (Thom et Church), a fungal pathogen of Caribbean sea fan corals. Hydrobiologia 460:105-11CrossRef
  Amend AS, Barshis DJ, Oliver TA (2012) Coral-associated marine fungi form novel lineages and heterogenous assemblages. ISME J 6:1291-301PubMed Central CrossRef PubMed
  Appleton DR, Copp BR (2003) Kottamide E, the first example of a natural product bearing the amino acid 4-amino-1, 2-dithiolane-4-carboxylic acid (Adt). Tetrahedron Lett 44:8963-965CrossRef
  Appleton DR, Page MJ, Lambert G, Berridge MV, Copp BR (2002) Kottamides A–D: novel bioactive imidazolone-containing alkaloids from the New Zea- land ascidian Pycnoclavella kottae. J Org Chem 67:5402-404CrossRef PubMed
  Blunt JW, Copp BR, Keyzers RA, Munro MHG, Prinsep MR (2014) Marine natural products. Nat Prod Rep 31:160-58CrossRef PubMed
  Blunt JW, Copp BR, Keyzers RA, Munro MHG, Prinsep MR (2015) Marine natural products. Nat Prod Rep 32:116-11CrossRef PubMed
  Bontemps N, Bry D, López-Legentil S, Simon-Levert A, Long C, Banaigs B (2010) Structures and antimicrobial activities of pyridoacridine alkaloids isolated from different chromotypes of the ascidian Cystodytes dellechiajei. J Nat Prod 73:1044-048CrossRef PubMed
  Bourne DG, Garren M, Work TM, Rosenberg E, Smith GW, Harvell CD (2009) Microbial disease and the coral holobiont. Trends Microbiol 17:554-62CrossRef PubMed
  Bry D, Banaigs B, Long C, Bontemps N (2011) New pyridoacridine alkaloids from the purple morph of the ascidian Cystodytes dellechiajei. Tetrahedron Lett 52:3041-044CrossRef
  Carlucci A, Raimondo ML, Santos JC, Phillips AJL (2012) Plectosphaerella species associated with root and collar rots of horticultural crops in southern Italy. Persoonia 28:34-8PubMed Central CrossRef PubMed
  Carman MR, Morris JA, Karney RC, Grunden DW (2010) An initial assessment of native and invasive tunicates in shellfish aquaculture of the North American east coast. J Appl Ichthyol 26:8-1CrossRef
  Carr G, Tay W, Bottriell H, Andersen SK, Mauk AG, Andersen RJ (2009) Plectosphaeroic acids A, B, and C, Indoleamine 2,3-Dioxygenase inhibitors produced in culture by a marine isolate of the fungus Plectosphaerella cucumerina. Org Lett 11:2996-999CrossRef PubMed
  Cuenca-Estrella M, Gomez-Lopez A, Mellado E, Buitrago MJ, Monzón A, Rodriguez-Tudela JL (2003) Scopulariopsis brevicaulis, a fungal pathogen resistant to broad-spectrum antifungal agents. Antimicrob Agents Chemother 47:2339-341PubMed Central CrossRef PubMed
  Dhar J, Carey PB (1993) Scopulariopsis brevicaulis skin lesions in an AIDS patient. AIDS 7:1283-284CrossRef PubMed
  Easson CG, Thacker RW (2014) Phylogenetic signal in the community structure of host-specific microbiomes of tropical marine sponges. Front Microbiol 5:Article 532CrossRef PubMed
  Ein-Gil N, Ilan M, Carmeli S, Smith GW, Pawlik JR, Yarden O (2009) Presence of Aspergillus sydowii, a pathogen of gorgonian sea fans in the marine sponge Spongia obscura. ISME J 3:752-55CrossRef PubMed
  Elad Y, Williamson B, Tudzynski P, Delen N (2007) Botrytis spp. and diseases they cause in agricultural systems—an Introduction. In: Elad Y, Williamson B, Tudzynski P, Delen N (eds) Botrytis: biology, pathology and control. Springer, The NetherlandsCrossRef
  Erwin PM, López-Legentil S, Schuhmann PW (2010) The pharmaceutical value of marine biodiversity for anti-cancer drug discovery. Ecol Econ 70:445-51CrossRef
  Erwin PM, Pita L, López-Legentil S, Turon X (2012) Stability of sponge-associated bacteria over large seasonal shifts in temperature and irradiance. Appl Environ Microbiol 78:7358-360PubMed Central CrossRef PubMed
  Erwin PM, Pineda MC, Webster N, Turon X, López-Legentil S (2013) Small core communities and high variability in bacteria associated with the introduced ascidian Styela plicata. Symbiosis 59:35-6CrossRef
  Erwin PM, Pineda MC, Webster NS, Turon X, López-Legentil S (2014) Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians. ISME J 8:575-88PubMed Central CrossRef PubMed
  Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-91CrossRef
  Geiser DM, Taylor JW, Ritchie KB, Smith GW (1998) Cause of sea fan death in the West Indies. Nature 394:137-38CrossRef
  H?ller U, Wright AD, Matthée GF, Konig GM, Draeger S, Aust H-J, Schulz B (2000) Fungi from marine sponges: diversity, biological activity and secondary me
• 作者单位：Susanna López-Legentil (1)
  Patrick M. Erwin (1)
  Marta Turon (2)
  Oded Yarden (3)
  
  1. Department of Biology and Marine Biology, and Center for Marine Science, University of North Carolina Wilmington, 5600 Marvin K. Moss Lane, Wilmington, NC, 28409, USA
  2. Center for Advanced Studies of Blanes (CEAB-CSIC), Accés Cala S. Francesc 14, 17300, Blanes, Girona, Spain
  3. Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
  
• 刊物主题：Evolutionary Biology; Developmental Biology; Microbiology; Plant Sciences; Ecology;
• 出版者：Springer Netherlands
• ISSN：1878-7665

文摘

Ascidians are known to harbor diverse and host-specific bacterial and archaeal communities in their tunic. However, to date, only one ascidian species has been investigated to assess symbiotic relationships with fungi and the extent of their diversity. In this study, we isolated and identified 37 strains of fungi in association with three common ascidian species in the NW Mediterranean Sea: Cystodytes dellechiajei, Didemnum fulgens, and Pycnoclavella communis, and 15 additional strains from concentrated seawater samples collected around the animals. Most of the isolated fungi were classified within four orders: Eurotiales (predominantly Penicillium spp.), Pleosporales, Hypocreales (predominantly Trichoderma spp.), and Capnodiales (Cladosporium spp.). Three additional fungal isolates from C. dellechiajei and D. fulgens belonged to the orders Helotiales, Phylachorales and Microascales, and matched to well-known plant and human pathogens (Botrytis cinerea, Plectosphaerella cucumerina and Scopulariopsis brevicaulis). Host-specificity of ascidian-associated fungi was not apparent and thus the significance of ascidian-fungal associations for ascidian wellbeing and their possible ecological roles remain unknown. Keywords Fungus Mediterranean Sea ITS Sea-squirt Tunicata Pathogens