Microsatellite markers for the endangered orchids Cattleya labiata Lindl. and C. warneri T. Moore (Orchidaceae)

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• 作者：Paulo Ricardo M. Almeida (1)
  M. Cristina López-Roberts (1)
  Bianca B. Zanotto Vigna (2)
  Anete P. Souza (3)
  Aristóteles Góes-Neto (1)
  Cássio van den Berg (1)
  
• 关键词：Cattleya ; SSR ; Genetic diversity ; Conservation genetics
• 刊名：Conservation Genetics Resources
• 出版年：2013
• 出版时间：September 2013
• 年：2013
• 卷：5
• 期：3
• 页码：791-794
• 全文大小：168KB
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• 作者单位：Paulo Ricardo M. Almeida (1)
  M. Cristina López-Roberts (1)
  Bianca B. Zanotto Vigna (2)
  Anete P. Souza (3)
  Aristóteles Góes-Neto (1)
  Cássio van den Berg (1)
  
  1. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Av. Transnordestina s.n., Feira de Santana, Bahia, 44036-900, Brazil
  2. Embrapa Southeast Livestock, CP 339, S?o Carlos, S?o Paulo, 13560-970, Brazil
  3. Centro de Biologia Molecular e Engenharia Genética (CBMEG) e Instituto de Biologia, Universidade Estadual de Campinas, CP 6010, Campinas, S?o Paulo, 13083-970, Brazil
  

文摘

In this study, we developed a new set of microsatellite markers for endangered Cattleya labiata and Cattleya warneri. Also the first microsatellite loci for Cattleya, these markers can be useful for studies in the conservation genetics of these species. Twenty-one new microsatellite primers pairs were characterized by cloning an enriched library, and two libraries were obtained by cloning inter-simple sequence repeats. Thirteen polymorphic primers were found, and the remaining eight were monomorphic for both species. Our results indicate that these new microsatellite markers have the potential to be used as a tool for studying the genetic diversity of C. labiata and C. warneri and for future studies focusing mating systems and the detection of hybrids.