γδ Τ cells enhance B cells for antibody production in Hashimoto's thyroiditis, and retinoic acid induces apoptosis of the γδ Τ cell

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• 作者：Hongli Liu ; Tingting Zheng ; Yufei Mao ; Chengcheng Xu ; Fei Wu ; Ling Bu…
• 关键词：Hashimoto’s thyroiditis ; γδ T cells ; All ; trans retinoic acid ; Autoantibodies
• 刊名：Endocrine
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：51
• 期：1
• 页码：113-122
• 全文大小：1,516 KB
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• 作者单位：Hongli Liu (1) (2) (3)
  Tingting Zheng (1) (2)
  Yufei Mao (4)
  Chengcheng Xu (1) (2)
  Fei Wu (1) (2)
  Ling Bu (1) (2)
  Xiao Mou (1) (2)
  Yuepeng Zhou (1) (2)
  Guoyue Yuan (1)
  Shengjun Wang (4)
  Tong Zhou (5)
  Deyu Chen (1) (2)
  Chaoming Mao (1) (2)
  
  1. Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, 212001, China
  2. Institute of Oncology, The Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, 212001, China
  3. Department of Laboratory Medicine, Nantong Tumor Hospital, Nantong, 226361, China
  4. Department of Laboratory Immunology, Jiangsu University School of Medicine, Zhenjiang, 212001, China
  5. Department of Pediatrics, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, China
  
• 刊物主题：Endocrinology; Diabetes; Internal Medicine; Science, general;
• 出版者：Springer US
• ISSN：1559-0100

文摘

TCR γδ+ Τ cells are important in the pathogenesis of inflammatory and autoimmune conditions. This study investigated the effect of γδ T cells on autoantibody production in patients with Hashimoto’s thyroiditis (HT). A total of 148 subjects were enrolled, including 99 patients with HT, 5 with simple goiters, and 44 healthy controls. Peripheral blood and thyroid mononuclear cells were subjected to flow cytometric analysis. Thyroid tissues underwent immunofluorescent staining and immunohistochemistry for γδ T cells and anti-thyroid antibody detection. Antibody production was measured by ELISA and automated chemiluminescent immunoassays. And activation and apoptosis of peripheral blood γδT cells and B cells were measured by flow cytometric analysis. The percentage of γδ T cells were greater in thyroid tissue from HT patients than that of goiter patients (n = 5, 5.33 ± 1.20 vs. 2.07 ± 0.44 %; P < 0.05), with the Vδ1+ γδ T cell subset especially dominant. Frequencies of CD69 (8.42 ± 1.08 vs. 1.60 ± 0.38 %, P < 0.001), HLA-DR (58.12 ± 6.36 vs. 37.82 ± 3.70 %, P < 0.05), CD40L (1.58 ± 0.35 vs. 0.15 ± 0.05 %, P < 0.01), and ICOS (2.78 ± 0.66 vs. 0.28 ± 0.13 %, P < 0.01) expressed on γδ T cells from HT patients (n = 19) were significantly increased compared with those of healthy controls (n = 15). More importantly, γδ T cells from HT patients enhanced B cells for antibody production, and all-trans retinoic acid (ATRA) treatment inhibited the effect by inducing apoptosis of γδ Τ cells. γδ Τ cells appear to play an important role in the pathogenesis of HT, and ATRA might be an effective regulator for HT patients.