Predominance of Ehrlichia chaffeensis in Rhipicephalus sanguineus ticks from kennel-confined dogs in Limbe, Cameroon

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• 作者：Lucy M. Ndip (1)
  Roland N. Ndip (2)
  Seraphine N. Esemu (1)
  David H. Walker (3) (4)
  Jere W. McBride (3) (4) (5)
  
• 关键词：Rhipicephalus sanguineus ; Ehrlichia chaffeensis ; Ehrlichia canis ; Zoonosis ; Cameroon
• 刊名：Experimental and Applied Acarology
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：50
• 期：2
• 页码：163-168
• 全文大小：200KB
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• 作者单位：Lucy M. Ndip (1)
  Roland N. Ndip (2)
  Seraphine N. Esemu (1)
  David H. Walker (3) (4)
  Jere W. McBride (3) (4) (5)
  
  1. Department of Biochemistry and Microbiology, University of Buea, P. O. Box 63, Buea, Cameroon
  2. Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa
  3. Department of Pathology, University of Texas Medical Branch, Galveston, TX, 77555-0609, USA
  4. Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX, 77555-0609, USA
  5. Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, 77555-0609, USA
  

文摘

Rhipicephalus sanguineus ticks (n?=?63) collected from five dogs (two adults and three puppies) housed in a kennel were screened for Ehrlichial agents (Ehrlichia canis, E. chaffeensis, and E. ewingii) using a species-specific multicolor real-time TaqMan PCR amplification of the disulphide bond formation protein (dsb) gene. Ehrlichia chaffeensis DNA was detected in 33 (56%) ticks, E. canis DNA was detected in four (6%) ticks, and one tick was coinfected. The E. chaffeensis and E. canis nucleotide sequences of the amplified dsb gene (374?bp) obtained from the Cameroonian R. sanguineus ticks were identical to the North American genotypes.