Protection of hepatic cell damage and antimicrobial evaluation of chitosan-catechin conjugate

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• 作者：Young-Sook Cho (1)
  Dae-Sung Lee (2)
  Young-Mog Kim (3)
  Chang-Bum Ahn (4)
  Do-Hyung Kim (5)
  Won-Kyo Jung (6)
  Jae-Young Je (1)
  
• 关键词：Antimicrobial ; Antioxidant ; Catechin ; Chitosan ; Hepatotoxicity ; Oxidative stress
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：56
• 期：6
• 页码：701-707
• 全文大小：245 KB
• 作者单位：Young-Sook Cho (1)
  Dae-Sung Lee (2)
  Young-Mog Kim (3)
  Chang-Bum Ahn (4)
  Do-Hyung Kim (5)
  Won-Kyo Jung (6)
  Jae-Young Je (1)
  
  1. Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu, 550-749, Republic of Korea
  2. POSTECH Ocean Science and Technology Institute, POSTECH, Pohang, 792-784, Republic of Korea
  3. Department of Food Science and Technology, Pukyong National University, Busan, 608-737, Republic of Korea
  4. Division of Food and Nutrition, Chonnam National University, Gwangju, 550-757, Republic of Korea
  5. Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, Republic of Korea
  6. Department of Biomedical Engineering, Center for Marine-Integrated Biomedical Technology (BK21 Plus) Pukyong National University, Busan, 608-737, Republic of Korea
  
• ISSN：2234-344X

文摘

The chitosan-catechin conjugate was developed by free radical-induced conjugating reaction, and its protection ability against hydrogen peroxide-induced hepatic damage in human normal Chang liver cells and antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and foodborne pathogens were investigated. Treatment of hydrogen peroxide (650 μM) on Chang liver cells decreased cell viability up to 59.38% compared to the non-treatment group; however, cotreatment of the chitosan-catechin conjugate increased cell viability up to 76.90% at 200 μg/mL, and the protection ability was significantly higher than the unmodified chitosan (p <0.05). The chitosan-catechin conjugate significantly (p <0.05) inhibited the formation of intracellular reactive oxygen species and lipid peroxidation in Chang liver cells. Moreover, the chitosan-catechin conjugate increased glutathione levels in normal condition as well as under oxidative stress by hydrogen peroxide. Additionally, the chitosan-catechin conjugate showed increased antimicrobial activity against MRSA and foodborne pathogens as compared to those of the unmodified chitosan.