Response to water deficit in glume of wheat: expression profiling by microarray analysis

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• 作者：Changxin Liu ; Huicong Mi ; Huawei Liu ; Shuangze Xie ; Yongjun Wu ; Pan Ning…
• 关键词：Triticum aestivum L. ; Glume ; Water deficit ; Microarray analysis ; qRT ; PCR
• 刊名：Euphytica
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：213
• 期：1
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Pathology; Plant Physiology; Biotechnology;
• 出版者：Springer Netherlands
• ISSN：1573-5060
• 卷排序：213

文摘

To investigate the drought response in wheat spike, we evaluated variations in gene expression in the early grain-filling period at 6 days after anthesis by Affymetrix Wheat Genome Array. 566 differentially expressed probe sets were identified. 477 genes were upregulated (FC ≥ 2.0) and 89 (FC ≤ 0.5) were downregulated under water deficit. Most upregulated genes were involved in signal transduction, metabolism, and transcription. We identified signaling proteins, transcription factors, and abiotic stress-related genes among the upregulated genes. Three important genes were located at the center of the phenylalanine metabolism. This result indicates that phenylalanine metabolism and biosynthesis of flavonoids derived from carbohydrate metabolism play pivotal roles during drought-resistance response in wheat spike. Nine upregulated genes were selected for confirming gene chip results at 0, 3, 6, 10, 15, 20, and 25 DAA by real-time PCR. This study provided insights into the molecular mechanism underlying wheat spike responses against drought.