Enhanced immunomagnetic separation for the detection of norovirus using the polyclonal antibody produced with human norovirus GII.4-like particles

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• 作者：Kangbum Lee (1) (2)
  Kwisung Park (3)
  Dong Joo Seo (1)
  Min Hwa Lee (1)
  Ji-Youn Jung (4)
  Gyung Jin Park (5)
  Dasom Yoon (6)
  Ki Hwan Park (6)
  Changsun Choi (1)
  
• 关键词：norovirus ; immunomagnetic separation ; virus ; like particles (VLPs) ; polyclonal antibody ; concentration
• 刊名：Food Science and Biotechnology
• 出版年：2014
• 出版时间：October 2014
• 年：2014
• 卷：23
• 期：5
• 页码：1569-1576
• 全文大小：248 KB
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• 作者单位：Kangbum Lee (1) (2)
  Kwisung Park (3)
  Dong Joo Seo (1)
  Min Hwa Lee (1)
  Ji-Youn Jung (4)
  Gyung Jin Park (5)
  Dasom Yoon (6)
  Ki Hwan Park (6)
  Changsun Choi (1)
  
  1. Department of Food and Nutrition, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea
  2. Gyeonggi-Do Institute of Public Health and Environment, Suwon, Gyeonggi, 442-701, Korea
  3. Chungcheongnam-Do Health and Environment Research Institute, Daejeon, 300-801, Korea
  4. Department of Companion and Laboratory Animal Science, Kongju National University, Kongju, Chungnam, 314-701, Korea
  5. Department of Food and Nutrition, Kunsan National University, Gunsan, Jeonbuk, 573-701, Korea
  6. Department of Food Science and Technology, Chung-Ang University, Anseong, Gyeonggi, 456-756, Korea
  
• ISSN：2092-6456

文摘

Human norovirus (NoV) is the most common cause of foodborne viral gastroenteritis worldwide. This study was aimed to develop the enhanced immunomagnetic separation (IMS) for effectively concentrating and detecting human noroviruses in food matrix. Virus-like particles (VLPs) were made by integrating NoV GII.4 capsid gene into baculovirus vector. In order to increase the sensitivity and specificity of immunomagnetic complex, polyclonal rabbit antibody against NoV GII.4 capsid was produced and used for producing immunomagnetic beads. IMS, polyethylene glycol precipitation, and ultrafiltration were compared to concentrate NoV spiked in vegetables. IMS was the most efficient method for concentrating NoV. Therefore, IMS developed in this study is the most effective method to concentrate and detect NoV contaminated in produce.