Improvements of metabolites tolerance in Clostridium acetobutylicum by micronutrient zinc supplementation

详细信息    查看全文

• 作者：You-Duo Wu ; Chuang Xue ; Li-Jie Chen…
• 关键词：Clostridium acetobutylicum ; formic acid ; acetic acid ; butyric acid ; butanol ; metabolites tolerance
• 刊名：Biotechnology and Bioprocess Engineering
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：21
• 期：1
• 页码：60-67
• 全文大小：404 KB
• 参考文献：1.Guo, M. X. W. P. Song, and J. Buhain (2015) Bioenergy and biofuels: History, status, and perspective. Renew. Sust. Energ. Rev. 42: 712–725.CrossRef
  2.Xue, C., X. Q. Zhao, C. G. Liu, L. J. Chen, and F. W. Bai (2013) Prospective and development of butanol as an advanced biofuel. Biotechnol. Adv. 31: 1575–1584.CrossRef
  3.Jones, D. T. and D. R. Woods (1986) Acetone-butanol fermentation revisited. Microbiol. Rev. 50: 484–524.
  4.Ezeji, T., C. Milne, N. D. Price, and H. P. Blaschek (2010) Achievements and perspectives to overcome the poor solvent resistance in acetone and butanol-producing microorganisms. Appl. Microbial. Biot. 85: 1697–1712.CrossRef
  5.Gheshlaghi, R., J. M. Scharer, M. Moo-Young, and C. P. Chou (2009) Metabolic pathways of clostridia for producing butanol. Biotechnol. Adv. 27: 764–781.CrossRef
  6.Wang, S.H., Y. P. Zhang, H. J. Dong, S. M. Mao, Y. Zhu, R. J. Wang, G. D. Luan, and Y. Li (2011) Formic acid triggers the "acid crash" of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. Appl. Environ. Microbiol. 77: 1674–1680.CrossRef
  7.Cho, D. H., S. J. Shin, and Y. H. Kim (2012) Effects of acetic and formic acid on ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Biotechnol. Bioproc. Eng. 17: 270–275.CrossRef
  8.Baral, N. R. and A. Shah (2014) Microbial inhibitors: formation and effects on acetone-butanol-ethanol fermentation of lignocellulosic biomass. Appl. Microbiol. Biot. 98: 9151–9172.CrossRef
  9.Tomas, C. A., J. Beamish, and E. T. Papoutsakis (2004) Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum. J. Bacteriol. 186: 2006–2018.CrossRef
  10.Alsaker, K. V., C. Paredes, and E. T. Papoutsakis (2010) Metabolite stress and tolerance in the production of biofuels and chemicals: Gene-expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum. Biotechnol. Bioeng. 105: 1131–1147.
  11.Borden, J. R., S. W. Jones, D. Indurthi, Y. L. Chen, and E. T. Papoutsakis (2010) A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing. Metab. Eng. 12: 268–281.CrossRef
  12.Venkataramanan, K. P., S. W. Jones, K. P. McCormick, S. G. Kunjeti, M. T. Ralston, B. C. Meyers, and E. T. Papoutsakis (2013) The Clostridium small RNome that responds to stress: the paradigm and importance of toxic metabolite stress in C. acetobutylicum. BMC Genom. 14: 849–864.CrossRef
  13.Xue, C., J. B. Zhao, L. J. Chen, F. W. Bai, S. T. Yang, and J. X. Sun (2014) Integrated butanol recovery for an advanced biofuel: Current state and prospects. Appl. Microbiol. Biot. 98: 3463–3474.CrossRef
  14.Xue, C., G. Q. Du, J. X. Sun, L. J. Chen, S. S. Gao, M. L. Yu, S. T. Yang, and F. W. Bai (2014) Characterization of gas stripping and its integration with acetone-butanol-ethanol fermentation for high-efficient butanol production and recovery. Biochem. Eng. J. 83: 55–61.CrossRef
  15.Heluane, H., M. R. Evans, S. F. Dagher, and J. M. Bruno-Barcena (2011) Meta-analysis and functional validation of nutritional requirements of solventogenic clostridia growing under butanol stress conditions and coutilization of D-glucose and D-xylose. Appl. Environ. Microbiol. 77: 4473–4485.CrossRef
  16.Li, J. Z., N. R. Baral, and A. K. Jha (2014) Acetone-butanol-ethanol fermentation of corn stover by Clostridium species: Present status and future perspectives. World J. Microb. Biot. 30: 1145–1157.CrossRef
  17.Wu, Y. D., C. Xue, L. J. Chen, and F. W. Bai (2013) Effect of zinc supplementation on acetone-butanol-ethanol fermentation by Clostridium acetobutylicum. J. Biotechnol. 165: 18–21.CrossRef
  18.Husemann, M. H. W. and E. T. Papoutsakis (1990) Effects of propionate and acetate additions on solvent production in batch cultures of Clostridium acetobutylicum. Appl. Environ. Microbiol. 56: 1497–1500.
  19.Chen, C. K. and H. P. Blaschek (1999) Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101. Appl. Microbiol. Biot. 52: 170–173.CrossRef
  20.Cho, D. H., Y. J. Lee, Y. Um, B. I. Sang, and Y. H. Kim (2009) Detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from Clostridium beijerinckii. Appl. Microbiol. Biot. 83: 1035–1043.CrossRef
  21.Lehmann, D., N. Radomski, and T. Lütke-Eversloh (2012) New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl. Microbiol. Biot. 96: 1325–1339.CrossRef
  22.Bergstrom, S. L. and G. L. Foutch (1983) Effect of cocultures on the production of butanol by Clostridium Sp. Biotechnol. Bioeng. 13: 251–256.
  23.Bowles, L. K. and W. L. Ellefson (1985) Effects of butanol on Clostridium acetobutylicum. Appl. Environ. Microbiol. 50: 1165–1170.
  24.Bankar, S. B., S. A. Survase, H. Ojamo, and T. Granstrom (2013) Biobutanol: The outlook of an academic and industrialist. RSC Adv. 3: 24734–24757.CrossRef
  25.Zheng, J., Y. Tashiro, Q. H. Wang, and K. Sonomoto (2015) Recent advances to improve fermentative butanol production: Genetic engineering and fermentation technology. J. Biosci. Bioeng. 119: 1–9.CrossRef
  26.Wang, Q. H., K. P. Venkataramanan, H. Z. Huang, E. T. Papoutsakis, and C. H. Wu (2013) Transcription factors and genetic circuits orchestrating the complex, multilayered response of Clostridium acetobutylicum to butanol and butyrate stress. BMC Syst. Biol. 7: 120–136.CrossRef
  27.Zhao, X. Q., C. Xue, X. M. Ge, W. J. Yuan, J. Y. Wang, and F. W. Bai (2009) Impact of zinc supplementation on the improvement of ethanol tolerance and yield of self-flocculating yeast in continuous ethanol fermentation. J. Biotechnol. 139: 55–60.CrossRef
  28.Xue, C., X. Q. Zhao, and F. W. Bai (2010) Effect of the size of yeast flocs and zinc supplementation on continuous ethanol fermentation performance and metabolic flux distribution under very high concentration conditions. Biotechnol. Bioeng. 105: 935–944.
  
• 作者单位：You-Duo Wu (1)
  Chuang Xue (1)
  Li-Jie Chen (1)
  Wen-Jie Yuan (1)
  Feng-Wu Bai (2)
  
  1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116-024, China
  2. School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200-240, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  
• 出版者：The Korean Society for Biotechnology and Bioengineering
• ISSN：1976-3816

文摘

Micronutrient zinc is of great importance for acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum. The effect of zinc supplementation on toxic metabolites (formic, acetic, butyric acid and butanol) tolerance during ABE fermentation was investigated under various stress-shock conditions without pH control. Great improvements on cell growth, glucose utilization and butanol production were achieved. In the presence of 0.45 g/L formic acid, zinc contributed to 11.28 g/L butanol produced from 55.24 g/L glucose compared to only 5.27 g/L butanol from 29.49 g/L glucose in the control without zinc supplementation. More importantly, relatively higher levels of 7.5 g/L acetic acid, 5.5 g/L butyric acid and 18 g/L butanol could be tolerated by C. acetobutylicum with zinc supplementation while no fermentation was observed under the same stress-shock condition respectively, suggesting that the acids and butanol tolerance in C. acetobutylicum could be significantly facilitated by pleiotropic regulation of micronutrient zinc. Thus, this paper provides an efficient bioprocess engineering strategy for improving stress tolerance in Clostridium species.