Multiaxial Polarity Determines Individual Cellular and Nuclear Chirality

详细信息    查看全文

• 作者：Michael J. Raymond Jr. ; Poulomi Ray ; Gurleen Kaur…
• 关键词：Cell chirality ; Cell polarity ; Cell morphology ; Nuclear morphology
• 刊名：Cellular and Molecular Bioengineering
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：10
• 期：1
• 页码：63-74
• 全文大小：
• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering; Classical Mechanics; Continuum Mechanics and Mechanics of Materials; Biological and Medical Physics, Biophysics; Biomaterials; Cell Biology;
• 出版者：Springer US
• ISSN：1865-5033
• 卷排序：10

文摘

Intrinsic cell chirality has been implicated in the left–right (LR) asymmetry of embryonic development. Impaired cell chirality could lead to severe birth defects in laterality. Previously, we detected cell chirality with an in vitro micropatterning system. Here, we demonstrate for the first time that chirality can be quantified as the coordination of multiaxial polarization of individual cells and nuclei. Using an object labeling, connected component based method, we characterized cell chirality based on cell and nuclear shape polarization and nuclear positioning of each cell in multicellular patterns of epithelial cells. We found that the cells adopted a LR bias the boundaries by positioning the sharp end towards the leading edge and leaving the nucleus at the rear. This behavior is consistent with the directional migration observed previously on the boundary of micropatterns. Although the nucleus is chirally aligned, it is not strongly biased towards or away from the boundary. As the result of the rear positioning of nuclei, the nuclear positioning has an opposite chirality to that of cell alignment. Overall, our results have revealed deep insights of chiral morphogenesis as the coordination of multiaxial polarization at the cellular and subcellular levels.