Immunophenotypic, cytogenetic and clinical features of 192 AML patients in China

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• 作者：Haixia Tong (1)
  Chunwei Lu (2)
  Jihong Zhang (1)
  Zhuogang Liu (3)
  Yu Ma (1)
  
• 关键词：Acute myeloid leukemia ; Immunophenotype ; Flow cytometry ; Cytogenetics
• 刊名：Clinical and Experimental Medicine
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：9
• 期：2
• 页码：149-155
• 全文大小：341KB
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• 作者单位：Haixia Tong (1)
  Chunwei Lu (2)
  Jihong Zhang (1)
  Zhuogang Liu (3)
  Yu Ma (1)
  
  1. Laboratory of Hematology, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, 110022, Shenyang, China
  2. Section of Health Testing, College of Public Health, China Medical University, No. 92 Northsecond Road, Heping District, 110001, Shenyang, China
  3. Department of Hematology, Shengjing Hospital of China Medical University, No. 39 Huaxiang Road, Tiexi District, 110022, Shenyang, China
  

文摘

Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification and prognosis of leukaemia. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. Among the 192 patients enrolled in this study, 125 cases were also subjected to karyotype analysis by G-banding technology. We found that CD33, CD13, MPO and CD117 were the most commonly expressed antigens in AML. A combination of intensive autofluorescence, both CD34?/sup> and HLA-DR?/sup>, and high expression of CD13, CD33 and MPO had significant value for M3 diagnosis. CD14 was expressed only in M4 and M5, and both intensive positivity of CD64 and CD15 with high expression of HLA-DR may suggest great possibility for diagnosis of M5. Lymphoid markers expression was documented in 47.9% of the 192 AML cases analyzed. CD56 (26.0%) and CD7 (20.8%) were the most commonly expressed lymphoid markers in AML patients. Abnormal karyotypes were detected in 76 out of 125 (60.8%). Higher CD34 positivity was found in LymAg+ group (77.2%) than in LymAg?/sup> group (48.0%). Our results indicate that immunophenotype analysis was useful for AML diagnosis and classification and the immunophenotype did have relevance to the abnormal cytogenetic changes and clinical features in AML.