The role of angiotensin II, endothelin-1 and transforming growth factor-&bgr; as autocrine/paracrine mediators of stretch-induced cardiomyocyte hypertrophy

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• 作者：van Wamel ; Annemieke J.E.T. ; Ruwhof ; Cindy ; van der Valk Kokshoorn ; Lizette E.J.M. ; Schriern ; Peter I. ; van der Laarse ; Arnoud
• 关键词：stretch ; cardiomyocytes ; fibroblasts ; endothelial cells ; vascular smooth muscle cells ; gene expression ; autocrine/paracrine mediators ; hypertrophy
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2001
• 出版时间：2001
• 年：2001
• 卷：218
• 期：1/2
• 页码：113-124
• 全文大小：192 KB

文摘

Cardiac hypertrophy is a compensatory response of myocardial tissue upon increased mechanical load. Of the mechanical factors, stretch is rapidly followed by hypertrophic responses. We tried to elucidate the role of angiotensin II (AII), endothelin-1 (ET-1) and transforming growth factor-&bgr; (TGF-&bgr;) as autocrine/paracrine mediators of stretch-induced cardiomyocyte hypertrophy. We collected conditioned medium (CM) from stretched cardiomyocytes and from other stretched cardiac cells, such as cardiac fibroblasts, endothelial cells and vascular smooth muscle cells (VSMCs). These CMs were administered to stationary cardiomyocytes with or without an AII type 1 (AT$\_1$) receptor antagonist (losartan), an ET-1 type A (ET$\_A$) receptor antagonist (BQ610), or anti-TGF-&bgr; antibodies. By measuring the mRNA levels of the proto-oncogene c-fos and the hypertrophy marker gene atrial natriuretic peptide (ANP), the molecular phenotype of the CM-treated stationary cardiomyocytes was characterized.

Our results showed that c-fos and ANP expression in stationary cardiomyocytes was increased by AII release from cardiomyocytes that had been stretched for 60 min. Stretched cardiomyocytes, cardiac fibroblasts and endothelial cells released ET-1 which led to increased c-fos and ANP expression in stationary cardiomyocytes. ET-1 released by stretched VSMCs, and TGF-&bgr; released by stretched cardiac fibroblasts and endothelial cells, appeared to be paracrine mediators of ANP expression in stationary cardiomyocytes.

These results indicate that AII, ET-1 and TGF-&bgr; (released by cardiac and vascular cell types) act as autocrine/paracrine mediators of stretch-induced cardiomyocyte hypertrophy. Therefore, it is likely that in stretched myocardium the cardiomyocytes, cardiac fibroblasts, endothelial cells and VSMCs take part in intercellular interactions contributing to cardiomyocyte hypertrophy.